User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/05

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(Day 4)
(Procedure)
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* We followed our general procedure from [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/01/29|1/29]].  
* We followed our general procedure from [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/01/29|1/29]].  
* We prepared two different solutions, one with the correct KNO<sub>3</sub> concentration and one with the incorrect one. For details on incorrect and correct concentrations see [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/04|2/4]]
* We prepared two different solutions, one with the correct KNO<sub>3</sub> concentration and one with the incorrect one. For details on incorrect and correct concentrations see [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/04|2/4]]
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Table 1. Preparation of both reaction mixtures
Table 1. Preparation of both reaction mixtures
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'''FTIR and XRD sample Prep'''
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For details on this process and procedure please see [[User:Moira M. Esson/Notebook/CHEM-571/2014/02/05|Moira's notebook]]
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==DATA==
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*All samples prepared on [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/04|2/4]] were analyzed using UV/vis spectroscopy.
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*The samples were not centrifuged because the nanoparticles/fibers were removed from the solution in preparation for FTIR and XRD analysis prior to UV/vis analysis.
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<br>
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'''Figure 1.''' Corrected Absorbance Spectra of Hemoglobin:Magnetite nanoparticles/fibers
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<br>
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[[Image:Corrected Absorbance Spectra of Heme-Magnetite nanoparticles .png]]
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<br>
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*Strong absorbance peak around 302nm corresponds to the excess nitrate present from the magnetite synthesis. The weak absorbance peaks
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'''Figure 2.''' Corrected Absorbance Spectra of BSA:Magnetite nanoparticles/fibers
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<br>
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[[Image:Corrected Absorbance Spectra of BSA-Magnetite nanoparticles .png]]
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==Observations & Notes==
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*We were unable to run AAS as we do not have an Iron Lamp for the AA at this time. The reaction mixtures (minus the precipitants) have been stored for now.
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* Please see [[User:Moira M. Esson/Notebook/CHEM-571/2014/02/05|Moira's Notebook]] for observations on particle size and magnetic characteristics observed.
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Revision as of 10:07, 19 February 2014

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Day 4

  • GOALS:
  1. Prepare BSA with magnetite using the general protocol described on 1/28 and 1/29 for comparison. (oven and nitrogen)
  2. UV-Vis of samples prepared on 1/29 and 2/4 (Magnetite-BSA made under nitrogen and Magnetite-BSA in oven and Hemoglobin+magnetite made in oven and under nitrogen.
  3. UV Vis protocol described on 1/29 in Moira’s Open wet ware
  4. Prepare all the magnetite samples from last week for FTIR and X-ray analysis.

Procedure

Preparation of BSA-Magnetitie nanoparticles

  • We followed our general procedure from 1/29.
  • We prepared two different solutions, one with the correct KNO3 concentration and one with the incorrect one. For details on incorrect and correct concentrations see 2/4

Table 1. Preparation of both reaction mixtures

Ratio concentration BSA (M) volume of BSA added (mL) amount of FeSO4 added (mL) amount of KOH added (mL) amount of KNO3 added (mL) volume water added (mL)
130002.30769E-060.4592100410.50.5006245542.51.040165405


Table 2. Preparation of BSA stock solution

Preparation of BSA stock '
Molecular weight (g/mol)66463
mass (g)0.0167
volume stock (L)0.01
Concentration (M)2.51268E-05


Table 3. Preparation of FeSO4*7H2O stock solution

Preparation of FeSO4 stock '
Molecular Weight (g/mol)278.01
mass(g)0.83403
volume stock (L)0.01
Concentration (M)0.3


Table 4. Preparation of KOH stock solution

Preparation of KOH stock '
Molecular Weight (g/mol)56.11
mass(g)0.5604
volume stock (L)0.01
Concentration (M)0.998752451


Table 5. Preparation of KNO3 stock solution for Reaction Mixture 1

Preparation of KNO3 '
Molecular Weight (g/mol)101.1
mass(g)4.044
volume stock (L)0.05
Concentration (M)0.8


Table 6. Preparation of KNO3 stock solution for Reaction Mixture 2

Preparation of KNO3 '
Molecular Weight (g/mol)101.1
mass(g)0.5604
volume stock (L)0.01
Concentration (M)0.5

FTIR and XRD sample Prep For details on this process and procedure please see Moira's notebook

DATA

  • All samples prepared on 2/4 were analyzed using UV/vis spectroscopy.
  • The samples were not centrifuged because the nanoparticles/fibers were removed from the solution in preparation for FTIR and XRD analysis prior to UV/vis analysis.


Figure 1. Corrected Absorbance Spectra of Hemoglobin:Magnetite nanoparticles/fibers
Image:Corrected Absorbance Spectra of Heme-Magnetite nanoparticles .png

  • Strong absorbance peak around 302nm corresponds to the excess nitrate present from the magnetite synthesis. The weak absorbance peaks

Figure 2. Corrected Absorbance Spectra of BSA:Magnetite nanoparticles/fibers
Image:Corrected Absorbance Spectra of BSA-Magnetite nanoparticles .png

Observations & Notes

  • We were unable to run AAS as we do not have an Iron Lamp for the AA at this time. The reaction mixtures (minus the precipitants) have been stored for now.
  • Please see Moira's Notebook for observations on particle size and magnetic characteristics observed.


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