User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/11

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(Procedure)
(Procedure)
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| 15000||0.000002||0.397982036||0.5||0.500624554||2.5||1.10139341
| 15000||0.000002||0.397982036||0.5||0.500624554||2.5||1.10139341
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*The second method we used was the Schlenk Line method- the same as was utilized on [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/01/29|1/29]]. 2 samples were made at the following concentrations
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Table 2: Hemoglobin stock solution
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Preparation of hemoglobin Stock solution'''
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| align="center" style="background:#f0f0f0;"|''''''
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| Molecular weight (g/mol)||64500
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|-
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| mass hemglobin (g)||0.0995
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| volume solution (L)||0.1
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| Concentration (M)||1.54264E-05
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|}
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Table 3: Hemoglobin- Magnetite solution ratios with Schlenk Method
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Ratio'''
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| align="center" style="background:#f0f0f0;"|'''concentration Hemoglobin (M)'''
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| align="center" style="background:#f0f0f0;"|'''volume of Hemoglobin added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''amount of FeSO4 added(mL)'''
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| align="center" style="background:#f0f0f0;"|'''amount of KOH added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''amount of KNO3 added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''volume Water added (mL)'''
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|-
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| 19000||1.57895E-06||0.511769373||0.5||0.500624554||2.5||0.987606073
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| 21000||1.42857E-06||0.463029433||0.5||0.500624554||2.5||1.036346013
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Revision as of 15:07, 24 February 2014

Project name Main project page
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Day 5

  • Goals
    • UV-Vis the reaction mixtures prepared on 2/5
    • Prepare a new ratio of BSA- Magnetite solutions using both the oven method and the Schlenk line method with which to prepare them. This ratio will be the same as the hemoglobin- magnetite ratios prepared with the Schlenk line.
    • Analyze the magnetite nanoparticles which were dried Last week using FTIR
    • Dry the particulate collected from the reaction mixtures prepared on 2/5 so that they may be used for FTIR and XRD next week.

Procedure

  • We prepared the stock solutions of FeSO4*7H2O ,KNO3, and KOH in the same method and at the same concentrations as prepared on February 4
  • We made the following ten ratios for the oven method. the same procedure as used on 1/29

Table 1: Preparation of BSA and Magnetite solutions.

Ratio concentration BSA (M) volume of BSA added (mL) amount of FeSO4 added(mL) amount of KOH added (mL) amount of KNO3 added (mL) volume Water added (mL)
105002.85714E-060.5685457660.50.5006245542.50.93082968
110002.72727E-060.5427027760.50.5006245542.50.95667267
115002.6087E-060.5191070030.50.5006245542.50.980268443
120000.00000250.4974775450.50.5006245542.51.001897901
125000.00000240.4775784430.50.5006245542.51.021797003
130002.30769E-060.4592100410.50.5006245542.51.040165405
135002.22222E-060.4422022620.50.5006245542.51.057173184
140002.14286E-060.4264093240.50.5006245542.51.072966122
145002.06897E-060.4117055540.50.5006245542.51.087669892
150000.0000020.3979820360.50.5006245542.51.10139341
  • The second method we used was the Schlenk Line method- the same as was utilized on 1/29. 2 samples were made at the following concentrations

Table 2: Hemoglobin stock solution

Preparation of hemoglobin Stock solution '
Molecular weight (g/mol)64500
mass hemglobin (g)0.0995
volume solution (L)0.1
Concentration (M)1.54264E-05

Table 3: Hemoglobin- Magnetite solution ratios with Schlenk Method

Ratio concentration Hemoglobin (M) volume of Hemoglobin added (mL) amount of FeSO4 added(mL) amount of KOH added (mL) amount of KNO3 added (mL) volume Water added (mL)
190001.57895E-060.5117693730.50.5006245542.50.987606073
210001.42857E-060.4630294330.50.5006245542.51.036346013

Data

For FTIR data and respective peaks please see Moira's Notebook


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