User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/12

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(Day 6)
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**UV-Vis the samples prepared on [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/11|Yesterday]]
**UV-Vis the samples prepared on [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/11|Yesterday]]
** Separate out the magnetite nanoparticles/ fibers using a magnet from the solutions prepared [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/11|Yesterday]] for future use in FTIR and XRD analysis
** Separate out the magnetite nanoparticles/ fibers using a magnet from the solutions prepared [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/11|Yesterday]] for future use in FTIR and XRD analysis
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**Analyze the magnetite nanoparticles/ fibers separated [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/11| Yesterday]] using  FTIR.  
+
**Analyze the magnetite nanoparticles/ fibers separated [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/11| Yesterday]] using  FTIR.
 +
 
 +
==Procedure==
 +
* We prepared the stock solutions of FeSO<sub>4</sub>*7H<sub>2</sub>O ,KNO<sub>3</sub>, and KOH in the same method and at the same concentrations as prepared on [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/04|February 4]]. The same hemoglobin stock solution as made [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/11| yesterday]] was used.
 +
* We made the following ten ratios for the oven method. the same procedure as used on [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/04|1/29]]
 +
 
 +
Table 1: Preparation of Reaction Mixtures for Hemeglobin- Magnetite Nanoparticle/Fiber formation
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{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Ratio'''
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| align="center" style="background:#f0f0f0;"|'''concentration Hemoglobin (M)'''
 +
| align="center" style="background:#f0f0f0;"|'''volume of Hemoglobin added (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''amount of FeSO4 added(mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''amount of KOH added (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''amount of KNO3 added (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''volume Water added (mL)'''
 +
|-
 +
| 10500||2.85714E-06||0.926058866||0.5||0.500624554||2.5||0.57331658
 +
|-
 +
| 11000||2.72727E-06||0.883965281||0.5||0.500624554||2.5||0.615410165
 +
|-
 +
| 11500||2.6087E-06||0.845532008||0.5||0.500624554||2.5||0.653843438
 +
|-
 +
| 12000||0.0000025||0.810301508||0.5||0.500624554||2.5||0.689073939
 +
|-
 +
| 12500||0.0000024||0.777889447||0.5||0.500624554||2.5||0.721485999
 +
|-
 +
| 13000||2.30769E-06||0.747970622||0.5||0.500624554||2.5||0.751404824
 +
|-
 +
| 13500||2.22222E-06||0.720268007||0.5||0.500624554||2.5||0.779107439
 +
|-
 +
| 14000||2.14286E-06||0.694544149||0.5||0.500624554||2.5||0.804831297
 +
|-
 +
| 14500||2.06897E-06||0.670594351||0.5||0.500624554||2.5||0.828781095
 +
|-
 +
| 15000||0.000002||0.648241206||0.5||0.500624554||2.5||0.85113424
 +
|}
 +
<br>
==Data==
==Data==

Revision as of 14:18, 24 February 2014

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Day 6

  • Goals
    • Prepare hemoglobin-magnetite solutions at the new ratios in the oven for comparison to the ones made of BSA. The BSA- Magnetite reaction mixtures at these ratios appeared to have more fiber like precipitates.
    • UV-Vis the samples prepared on Yesterday
    • Separate out the magnetite nanoparticles/ fibers using a magnet from the solutions prepared Yesterday for future use in FTIR and XRD analysis
    • Analyze the magnetite nanoparticles/ fibers separated Yesterday using FTIR.

Procedure

  • We prepared the stock solutions of FeSO4*7H2O ,KNO3, and KOH in the same method and at the same concentrations as prepared on February 4. The same hemoglobin stock solution as made yesterday was used.
  • We made the following ten ratios for the oven method. the same procedure as used on 1/29

Table 1: Preparation of Reaction Mixtures for Hemeglobin- Magnetite Nanoparticle/Fiber formation

Ratio concentration Hemoglobin (M) volume of Hemoglobin added (mL) amount of FeSO4 added(mL) amount of KOH added (mL) amount of KNO3 added (mL) volume Water added (mL)
105002.85714E-060.9260588660.50.5006245542.50.57331658
110002.72727E-060.8839652810.50.5006245542.50.615410165
115002.6087E-060.8455320080.50.5006245542.50.653843438
120000.00000250.8103015080.50.5006245542.50.689073939
125000.00000240.7778894470.50.5006245542.50.721485999
130002.30769E-060.7479706220.50.5006245542.50.751404824
135002.22222E-060.7202680070.50.5006245542.50.779107439
140002.14286E-060.6945441490.50.5006245542.50.804831297
145002.06897E-060.6705943510.50.5006245542.50.828781095
150000.0000020.6482412060.50.5006245542.50.85113424


Data


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