User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/12

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(Day 6)
(Data)
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==Observations==
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* For the oven prep reaction mixtures. It was noticed that immediately after adding the  FeSO<sub>4</sub>*7H<sub>2</sub>O a dark(dark green/blue) precipitate in both small clusters and long string like shape formed in the Heme:Magnetite samples. We always add FeSO<sub>4</sub>*7H<sub>2</sub>O last into the solutions so that it does not prematurely react with the other compounds and to make sure it does not inhibit the reaction from proceeding.
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*Upon observing the BSA:Magnetite samples, it appeared that large clusters formed (leaf like structures) at the 12500:1 ratio and continued to form up to the 15000:1 ratio. Prior to the 12500:1 ratio, it appears a magnetic colloid suspension was formed, indicating that all ratios successfully formed magnetite nanoparticles. However, for potential fiber formation, it appears the 12500:1-15000:1 range has potential.
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Revision as of 15:22, 24 February 2014

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Day 6

  • Goals
    • Prepare hemoglobin-magnetite solutions at the new ratios in the oven for comparison to the ones made of BSA. The BSA- Magnetite reaction mixtures at these ratios appeared to have more fiber like precipitates.
    • UV-Vis the samples prepared on Yesterday
    • Separate out the magnetite nanoparticles/ fibers using a magnet from the solutions prepared Yesterday for future use in FTIR and XRD analysis
    • Analyze the magnetite nanoparticles/ fibers separated Yesterday using FTIR.

Procedure

  • We prepared the stock solutions of FeSO4*7H2O ,KNO3, and KOH in the same method and at the same concentrations as prepared on February 4. The same hemoglobin stock solution as made yesterday was used.
  • We made the following ten ratios for the oven method. the same procedure as used on 1/29

Table 1: Preparation of Reaction Mixtures for Hemeglobin- Magnetite Nanoparticle/Fiber formation

Ratio concentration Hemoglobin (M) volume of Hemoglobin added (mL) amount of FeSO4 added(mL) amount of KOH added (mL) amount of KNO3 added (mL) volume Water added (mL)
105002.85714E-060.9260588660.50.5006245542.50.57331658
110002.72727E-060.8839652810.50.5006245542.50.615410165
115002.6087E-060.8455320080.50.5006245542.50.653843438
120000.00000250.8103015080.50.5006245542.50.689073939
125000.00000240.7778894470.50.5006245542.50.721485999
130002.30769E-060.7479706220.50.5006245542.50.751404824
135002.22222E-060.7202680070.50.5006245542.50.779107439
140002.14286E-060.6945441490.50.5006245542.50.804831297
145002.06897E-060.6705943510.50.5006245542.50.828781095
150000.0000020.6482412060.50.5006245542.50.85113424


Data

Observations

  • For the oven prep reaction mixtures. It was noticed that immediately after adding the FeSO4*7H2O a dark(dark green/blue) precipitate in both small clusters and long string like shape formed in the Heme:Magnetite samples. We always add FeSO4*7H2O last into the solutions so that it does not prematurely react with the other compounds and to make sure it does not inhibit the reaction from proceeding.
  • Upon observing the BSA:Magnetite samples, it appeared that large clusters formed (leaf like structures) at the 12500:1 ratio and continued to form up to the 15000:1 ratio. Prior to the 12500:1 ratio, it appears a magnetic colloid suspension was formed, indicating that all ratios successfully formed magnetite nanoparticles. However, for potential fiber formation, it appears the 12500:1-15000:1 range has potential.


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