User:Puja Mody/Notebook/AU Biomaterials 572/2014/04/16

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(DATA)
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==Goals==
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*View samples of Magnetite-Protein (made in oven) as well as magnetite- protein-gold samples made in the oven and via sonication.
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*Run DSC of samples dried out yesterday
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#13500 mag BSA gold N2 prep
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#13500 mag-BSA N2 prep
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*Run AA of remaining necessary samples (both Gold and Fe)
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==DATA==
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Table 1: Sonicated samples microscope data
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Sample Name 165 mag heme gold sonicated 2.5x
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Particle number pixel size actual size (mm) Actual size (m) Average
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1 34 0.079069767 7.90698E-05 9.81395E-05 98.13953488
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2 53 0.123255814 0.000123256
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3 26 0.060465116 6.04651E-05 STD DEV
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4 46 0.106976744 0.000106977 1.75338E-05 17.53378018
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5 43 0.1 0.0001
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6 46 0.106976744 0.000106977
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7 47 0.109302326 0.000109302
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8 42 0.097674419 9.76744E-05
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9 40 0.093023256 9.30233E-05
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10 45 0.104651163 0.000104651
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Sample name 130 mag bsa gold sonicated 2.5 x
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Particle number pixel size actual size (mm) Actual size (m) Average
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1 32 0.074418605 7.44186E-05 8.27907E-05 82.79069767
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2 43 0.1 0.0001
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3 37 0.086046512 8.60465E-05 STD DEV
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4 33 0.076744186 7.67442E-05 1.07526E-05 10.75258349
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5 30 0.069767442 6.97674E-05
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6 35 0.081395349 8.13953E-05
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7 37 0.086046512 8.60465E-05
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8 40 0.093023256 9.30233E-05
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9 29 0.06744186 6.74419E-05
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10 40 0.093023256 9.30233E-05
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Table 2: Oven prep samples microscope data
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Sample name'''
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| align="center" style="background:#f0f0f0;"|'''165 mag heme oven'''
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| align="center" style="background:#f0f0f0;"|''''''
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| align="center" style="background:#f0f0f0;"|''''''
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| align="center" style="background:#f0f0f0;"|''''''
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| align="center" style="background:#f0f0f0;"|''''''
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|-
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| Particle number||pixel size||actual size (mm)||Actual size (m)||Average||in um
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| 1||90||0.209302326||0.000209302||0.000163123||163.1229236
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| 2||66||0.153488372||0.000153488||||
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| 3||65||0.151162791||0.000151163||STD DEV||
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| 4||67||0.155813953||0.000155814||2.25645E-05||22.56447491
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| 5||61||0.141860465||0.00014186||||
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| 6||67||0.155813953||0.000155814||||
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| 7||75||0.174418605||0.000174419||||
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| ||||||||||
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|-
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| Fiber width range||pixel size ||actual size (mm)||actual size (m)||average||in um
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| 1||30||0.069767442||6.97674E-05||0.000202093||202.0930233
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| 2||110||0.255813953||0.000255814||||
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| 3||181||0.420930233||0.00042093||STD DEV||
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| 4||12||0.027906977||2.7907E-05||0.000160233||160.2325769
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| 5||81||0.188372093||0.000188372||||
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| 6||23||0.053488372||5.34884E-05||||
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| 7||147||0.341860465||0.00034186||||
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| 8||39||0.090697674||9.06977E-05||||
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| 9||46||0.106976744||0.000106977||||
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| 10||200||0.465116279||0.000465116||||
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| Sample Name||130 mag heme oven||||||||
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|-
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| Particle number ||pixel size ||actual size (mm)||actual size (m)||average||in um
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| 1||56||0.130231733||0.000130232||0.000295347||295.3469667
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| 2||226||0.525578067||0.000525578||||
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| 3||137||0.318602633||0.000318603||||
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| 4||49||0.113952767||0.000113953||STD DEV||
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| 5||200||0.465113333||0.000465113||0.000165655||165.6548003
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| 6||222||0.5162758||0.000516276||||
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| 7||132||0.3069748||0.000306975||||
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| 8||49||0.113952767||0.000113953||||
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| 9||63||0.1465107||0.000146511||||
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| 10||136||0.316277067||0.000316277||||
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| Fiber width range||pixel size ||actual size (mm)||actual size (m)||average||in um
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| 1||106||0.246510067||0.00024651||0.000219932||219.9321619
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| 2||185||0.430229833||0.00043023||||
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| 3||79||0.183719767||0.00018372||STD DEV||
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| 4||116||0.269765733||0.000269766||0.000110007||110.0073921
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| 5||76||0.176743067||0.000176743||||
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| 6||57||0.1325573||0.000132557||||
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| 7||43||0.099999367||9.99994E-05||||
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| Sample Name||135 mag BSA oven gold||||||||
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| Particle number ||pixel size ||actual size (mm)||actual size (m)||average||in um
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| 1||102||0.2372078||0.000237208||0.000202092||202.0917433
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| 2||85||0.197673167||0.000197673||||
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| 3||70||0.162789667||0.00016279||||
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| 4||132||0.3069748||0.000306975||STD DEV||
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| 5||83||0.193022033||0.000193022||8.66719E-05||86.67187257
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| 6||55||0.127906167||0.000127906||||
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| 7||30||0.069767||0.000069767||||
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| 8||125||0.290695833||0.000290696||||
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| 9||139||0.323253767||0.000323254||||
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| 10||48||0.1116272||0.000111627||||
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| Sample Name||165 mag heme oven gold||||||||
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| Particle number ||pixel size ||actual size (mm)||actual size (m)||average||in um
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| 1||126||0.2930214||0.000293021||0.000253022||253.0216533
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| 2||76||0.176743067||0.000176743||||
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| 3||88||0.204649867||0.00020465||STD DEV||
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| 4||120||0.279068||0.000279068||5.73933E-05||57.39330175
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| 5||83||0.193022033||0.000193022||||
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| 6||146||0.339532733||0.000339533||||
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| 7||134||0.311625933||0.000311626||||
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| 8||123||0.2860447||0.000286045||||
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| 9||109||0.253486767||0.000253487||||
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| 10||83||0.193022033||0.000193022||||
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|}
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Table 3: AA data for both gold and Iron Lamps
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Sample number'''
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| align="center" style="background:#f0f0f0;"|'''Sample name'''
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| align="center" style="background:#f0f0f0;"|'''Concentration Gold in sample (ug/mL)'''
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| align="center" style="background:#f0f0f0;"|'''Total concentration Gold originally in solution (ug/mL)'''
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| align="center" style="background:#f0f0f0;"|'''Concentration gold incorporated (ug/mL)'''
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| align="center" style="background:#f0f0f0;"|'''Percent Gold incorporated (%)'''
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| 1||13500:1 Mag:BSA N2 prep with Au||0.04467344||1765.35||1765.305327||99.99746943
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| 2||13500:1 Mag:BSA:Au sonicated||0.0464768||1765.35||1765.303523||99.99736728
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| 3||13000:1 Mag:BSA:Au oven||0.04685952||1765.35||1765.30314||99.9973456
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| 4||16500:1 Mag:Heme:Au oven||0.04447792||1765.35||1765.305522||99.9974805
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| 5||16500:1 Mag:Heme:Au sonicated||0.04400784||1765.35||1765.305992||99.99750713
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| Sample number||Sample name||Concentration Iron in sample (ug/mL)||Total concentration Iron originally in solution (ug/mL)||Concentration Iron incorporated (ug/mL)||Percent Iron incorporated (%)
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| 1||13500:1 Mag:BSA N2 prep||0.022256152||1765.35||1765.327744||99.99873928
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| 2||13500:1 Mag:BSA N2 prep with Au||0.022219961||1765.35||1765.32778||99.99874133
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| 3||13000:1 Mag:BSA oven||0.02224606||1765.35||1765.327754||99.99873985
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| 4||16500:1 Mag:Heme oven||0.02224084||1765.35||1765.327759||99.99874015
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| 5||16500:1 Mag:Heme:Au oven||0.022238231||1765.35||1765.327762||99.99874029
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| 6||16500:1 Mag:Heme:Au sonicated||0.022220831||1765.35||1765.327779||99.99874128
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| 7||13500:1 Mag:BSA:Au sonicated||0.022207781||1765.35||1765.327792||99.99874202
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| 8||13000:1 Mag:BSA:Au oven||0.022180812||1765.35||1765.327819||99.99874355
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|}
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