User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/08/29: Difference between revisions
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M<sub>1</sub>V<sub>1</sub>= M<sub>2</sub>V<sub>2</sub> | M<sub>1</sub>V<sub>1</sub>= M<sub>2</sub>V<sub>2</sub> | ||
7720μM · X= (1.5 × (y)) ×6mL Where y= the desired concentration of each test tube (60, 80, 100 ....etc) | 7720μM · X= (1.5 × (y)) ×6mL Where y= the desired concentration of each test tube (60, 80, 100 ....etc) | ||
* Once the appropriate concentrations of the solutions were mixed, the test tubes were wrapped in foil and placed in an oven at a temperature of 80°C for 4 hours in order to unfold and refold the protein around the AuNP. | * Once the appropriate concentrations of the solutions were mixed, the test tubes were wrapped in foil and placed in an oven at a temperature of 80°C for 4 hours in order to unfold and refold the protein around the AuNP. | ||
==Calculations== | |||
##m<sub>1</sub>v<sub>1</sub>=m<sub>2</sub>v<sub>2</sub> | |||
##15μM*(volume [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] stock | |||
solution) = (1.5*6) | |||
###0.6mL [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] stock | |||
solution in each tube | |||
##7720μM*x mL=6mL*(1.5*60μM) | |||
###0.069mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] | |||
stock solution | |||
###5.331 mL H<sub>2</sub>O | |||
##7720μM*x mL=6mL*(1.5*80μM) | |||
###0.093mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] | |||
stock solution | |||
###5.307mL H<sub>2</sub>O | |||
##7720μM*x mL=6mL*(1.5*100μM) | |||
###0.117mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] | |||
stock solution | |||
###5.283mL H<sub>2</sub>O | |||
##7720μM*x mL=6mL*(1.5*120μM) | |||
###0.134mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] | |||
stock solution | |||
###5.266mL H<sub>2</sub>O | |||
##7720μM*x mL=6mL*(1.5*128μM) | |||
###0.149mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] | |||
stock solution | |||
###5.251mL H<sub>2</sub>O | |||
##7720μM*x mL=6mL*(1.5*130μM) | |||
###.152mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] | |||
stock solution | |||
###5.248mL H<sub>2</sub>O | |||
##7720μM*x mL=6mL*(1.5*132μM) | |||
###0.154mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] | |||
stock solution | |||
###5.246mL H<sub>2</sub>O | |||
##7720μM*x mL=6mL*(1.5*133μM) | |||
###0.155mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] | |||
stock solution | |||
###5.245mL H<sub>2</sub>O | |||
##7720μM*x mL=6mL*(1.5*134μM) | |||
###0.156mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] | |||
stock solution | |||
###5.254mL H<sub>2</sub>O | |||
##7720μM*x mL=6mL*(1.5*136μM) | |||
###0.159mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] | |||
stock solution | |||
###5.244mL H<sub>2</sub>O | |||
##7720μM*x mL=6mL*(1.5*138μM) | |||
###0.161mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] | |||
stock solution | |||
###5.242mL H<sub>2</sub>O | |||
##7720μM*x mL=6mL*(1.5*140μM) | |||
###0.163mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] | |||
stock solution | |||
###5.237mL H<sub>2</sub>O | |||
##7720μM*x mL=6mL*(1.5*160μM) | |||
###0.187mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] | |||
stock solution | |||
###5.213mL H<sub>2</sub>O | |||
##7720μM*x mL=6mL*(1.5*170μM) | |||
###0.198mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] | |||
stock solution | |||
###5.202mL H<sub>2</sub>O | |||
#The tubes were wrapped in tin foil and heated at 80°C for 4 hours. | |||
==Results/Conclusions== | ==Results/Conclusions== |
Revision as of 10:43, 11 September 2012
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Day 1
Goals
Procedure
.376 μM x66430 g/mol (MW of BSA)= .0249g BSA in 25mL of water.
M1V1= M2V2 7720μM · X= (1.5 × (y)) ×6mL Where y= the desired concentration of each test tube (60, 80, 100 ....etc)
Calculations
solution) = (1.5*6)
solution in each tube
stock solution
stock solution
stock solution
stock solution
stock solution
stock solution
stock solution
stock solution
stock solution
stock solution
stock solution
stock solution
stock solution
stock solution
Results/Conclusions
.0656g x 1mol/339.79 (MW HAuCl4)= 1.93 x10-4 / .025L= .00772 M/L.
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