User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/04: Difference between revisions
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*The pH of 8 was chosen because the optimal pH range for Tris is between 7-9. The tris buffer was also made at a pH of 10 because that was the pH at which the samples had been previously tested by others. And since this portion of the experiment is based on the work of the previous class and our desire is to repeat and build upon, it seemed appropriate. | *The pH of 8 was chosen because the optimal pH range for Tris is between 7-9. The tris buffer was also made at a pH of 10 because that was the pH at which the samples had been previously tested by others. And since this portion of the experiment is based on the work of the previous class and our desire is to repeat and build upon, it seemed appropriate. | ||
* prior to the changing of the pH, the original pH of the tris buffer was slightly above pH 10 for each solution. | * prior to the changing of the pH, the original pH of the tris buffer was slightly above pH 10 for each solution. | ||
* The UV-Vis showed that several of the concentration samples had a similar peak at approximated 550nm. The spike is related to the how the light is passing through the AuNP- how the light responds to the NP. The peak position is related to the concentration of the AuNP fibers in the BSA solution. Thus, based on the data presented in the graph, it would seem that at the concentrations where the major peaks are similar, the NP in those samples are the same size. | |||
Revision as of 08:47, 11 September 2012
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Day 2Goals
Procedure
.1Μ/L Tris × .1L = .01 mols .01 mols × 121.14 g/M (mw tris) = 1.21 grams of tris
Conclusions/Results
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