User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/05: Difference between revisions

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* Remake certain AuNP/BSA solutions at specific concentrations to retest using UV-Vis to check/clarify discrepancies
* Remake certain AuNP/BSA solutions at specific concentrations to retest using UV-Vis to check/clarify discrepancies
*Centrifuge the gold solutions in order to separate the fibers and then combine the fibers with the Tris buffer at various concentrations in order to test how long it takes to (if it does) re-suspend in the solution.
*Centrifuge the gold solutions in order to separate the fibers and then combine the fibers with the Tris buffer at various concentrations in order to test how long it takes to (if it does) re-suspend in the solution.
==Procedure==
* The AuNP stock solution from yesterday was combined with a new stock solution of BSA. The concentrations that were retested were: 120, 128, 130, 132, 133, 134. These samples were then placed in the oven for four hours in order to allow for the BSA to unfold and to be wrapped around the AuNP.
* The AuNP solutions that has been used previously were meanwhile transferred from glass test tubes to plastic ones in order to prepare them for centrifuging. The centrifuging was necessary in order to separate out the fibers as best as possible from the solution.
* The AuNP fibers were centrifuged for five minutes at a speed of 3000 rpm at a temperature of 10°C.
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Revision as of 11:30, 5 September 2012

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Entry title

Goals

  • Remake certain AuNP/BSA solutions at specific concentrations to retest using UV-Vis to check/clarify discrepancies
  • Centrifuge the gold solutions in order to separate the fibers and then combine the fibers with the Tris buffer at various concentrations in order to test how long it takes to (if it does) re-suspend in the solution.

Procedure

  • The AuNP stock solution from yesterday was combined with a new stock solution of BSA. The concentrations that were retested were: 120, 128, 130, 132, 133, 134. These samples were then placed in the oven for four hours in order to allow for the BSA to unfold and to be wrapped around the AuNP.
  • The AuNP solutions that has been used previously were meanwhile transferred from glass test tubes to plastic ones in order to prepare them for centrifuging. The centrifuging was necessary in order to separate out the fibers as best as possible from the solution.
  • The AuNP fibers were centrifuged for five minutes at a speed of 3000 rpm at a temperature of 10°C.


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