User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/05: Difference between revisions

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* After these new solutions were made, they were placed in UV-Vis three times. each time to test if the fibers had been dissolved into the tris buffer solution.  
* After these new solutions were made, they were placed in UV-Vis three times. each time to test if the fibers had been dissolved into the tris buffer solution.  
*  
*  
==Data==
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Test Tube'''
| align="center" style="background:#f0f0f0;"|'''Tris Buffer (mL)'''
| align="center" style="background:#f0f0f0;"|'''Water (mL)'''
| align="center" style="background:#f0f0f0;"|'''Concentration of solution M'''
|-
| 1||10||0||.1 M
|-
| 1||1||9||1 x 10^-2 M
|-
| 1||1||9||1 x 10^-3 M
|-
| 1||1||9||1 x 10^-4 M
|-
| 1||1||9||1 x10^-5 M
|-
| 1||1||9||1 x 10 ^-6 M
|-
| 1||1||9||1 x 10^-7 M
|}
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|}


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Revision as of 09:00, 11 September 2012

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Day 3

Goals

  • Remake certain AuNP/BSA solutions at specific concentrations to retest using UV-Vis to check/clarify discrepancies
  • Centrifuge the gold solutions in order to separate the fibers and then combine the fibers with the Tris buffer at various concentrations in order to test how long it takes to (if it does) re-suspend in the solution.

Procedure

  • The AuNP stock solution from yesterday was combined with a new stock solution of BSA. The concentrations that were retested were: 120, 128, 130, 132, 133, 134. These samples were then placed in the oven for four hours in order to allow for the BSA to unfold and to be wrapped around the AuNP.
  • The AuNP solutions that has been used previously were meanwhile transferred from glass test tubes to plastic ones in order to prepare them for centrifuging. The centrifuging was necessary in order to separate out the fibers as best as possible from the solution.
  • The AuNP fibers were centrifuged for five minutes at a speed of 3000 rpm at a temperature of 10°C. After which, the BSA solution was pipetted out as best as was possible. 7 test tubes which had centrifuged out the most of the AuNP fibers were used for the next step.
  • A serial dilation was used inorder to place various concentrations of the tris buffer and water into the test tubes. The concentrations ranged from .1M to 1×10−2 to 1×10-7.
  • After centrifuging, the AuNP fibers were separated from solution by pipetting out as much of the aqueous solution as possible prior to adding the tris buffer.
  • After these new solutions were made, they were placed in UV-Vis three times. each time to test if the fibers had been dissolved into the tris buffer solution.

Data

Test Tube Tris Buffer (mL) Water (mL) Concentration of solution M
1 10 0 .1 M
1 1 9 1 x 10^-2 M
1 1 9 1 x 10^-3 M
1 1 9 1 x 10^-4 M
1 1 9 1 x10^-5 M
1 1 9 1 x 10 ^-6 M
1 1 9 1 x 10^-7 M




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