User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/11: Difference between revisions

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* More AuNP/ BSA solution was made at a concentration of 170 and place in the oven for 4 hours at 80°C. These samples are to be used tomorrow and will be combined with the pH 10 tris buffer solution and have a UV-Vis run on them every hour.  
* More AuNP/ BSA solution was made at a concentration of 170 and place in the oven for 4 hours at 80°C. These samples are to be used tomorrow and will be combined with the pH 10 tris buffer solution and have a UV-Vis run on them every hour.  


==Data==
==Data/ Calculations==
#Solution was made with a mole ratio of 170
##2.4268M BSA solution was used
###m<sub>1</sub>v<sub>1</sub>=m<sub>2</sub>v<sub>2</sub>
### 24.266μM * (volume) = 1μM * 6mL
### .247mL BSA solution
## 2150μM HAuCl4 solution was used
### 2150μM * (volume) = 6mL *(1μM*170)
### .474mL HAuCl<sub><sub>4</sub></sub> solution
 
 
 
Link to spreadsheet and chart of redone UV-Vis:
Link to spreadsheet and chart of redone UV-Vis:
*http://openwetware.org/wiki/Image:Chem-_Redone_AuNP-BSA.xlsx
*http://openwetware.org/wiki/Image:Chem-_Redone_AuNP-BSA.xlsx

Revision as of 10:53, 12 September 2012

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Day 4

Goals

  • Run UV-Vis on AuNP/BSA samples 120, 128, 130, 132, 133, 134. and see if similar peaks are present
  • Run third UV-Vis on Tris buffer solutions of pH 8 and compare absorbances to the data collected last week.

Procedure

  • UV-Vis was run for the AuNP/BSA samples as well as water to account for background noise.
  • The third UV-Vis was run on the Tris buffer solutions at pH 8
  • More AuNP/ BSA solution was made at a concentration of 170 and place in the oven for 4 hours at 80°C. These samples are to be used tomorrow and will be combined with the pH 10 tris buffer solution and have a UV-Vis run on them every hour.

Data/ Calculations

  1. Solution was made with a mole ratio of 170
    1. 2.4268M BSA solution was used
      1. m1v1=m2v2
      2. 24.266μM * (volume) = 1μM * 6mL
      3. .247mL BSA solution
    2. 2150μM HAuCl4 solution was used
      1. 2150μM * (volume) = 6mL *(1μM*170)
      2. .474mL HAuCl4 solution


Link to spreadsheet and chart of redone UV-Vis:

Link to spreadsheet and charts of the 3 UV-Vis data on the solutions of AuNP with tris buffer.

Conclusions

For the redone AuNP/BSA UV-Vis tests done. Instead of just one peak at 550nm, there was an uncharacteristic peak at around 490-500nm as well. As a result, distilled water was also tested to see if it had been a factor in causing an adjustment to the same. In the data spreadsheet, the UV-Vis data for each of the samples then had the UV-Vis data for water subtracted from it in order to test if it was the water that had caused the peak. The graphs clearly show that without the water, the background, the second peak was removed.

  • Other observations were that compared to the original 14 solution data, the absorbency was much higher as compared to the retested 6 samples where the absorbency is smalled by an entire order of magnitude.






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