User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/11: Difference between revisions
From OpenWetWare
No edit summary |
|||
Line 15: | Line 15: | ||
* UV-Vis was run for the AuNP/BSA samples as well as water to account for background noise. | * UV-Vis was run for the AuNP/BSA samples as well as water to account for background noise. | ||
*#UV/Vis was run on HAuCl<sub>4</sub> and BSA solution with mole ratios of 120, 128, 130, 132, 133 and 134. | *#UV/Vis was run on HAuCl<sub>4</sub> and BSA solution with mole ratios of 120, 128, 130, 132, 133 and 134. | ||
##The same cuvette was used and cleaned between each spectra. | *##The same cuvette was used and cleaned between each spectra. | ||
* The third UV-Vis was run on the Tris buffer solutions at pH 8 | * The third UV-Vis was run on the Tris buffer solutions at pH 8 | ||
* More AuNP/ BSA solution was made at a concentration of 170 and place in the oven for 4 hours at 80°C. These samples are to be used tomorrow and will be combined with the pH 10 tris buffer solution and have a UV-Vis run on them every hour. | * More AuNP/ BSA solution was made at a concentration of 170 and place in the oven for 4 hours at 80°C. These samples are to be used tomorrow and will be combined with the pH 10 tris buffer solution and have a UV-Vis run on them every hour. | ||
==Data/ Calculations== | ==Data/ Calculations== |
Revision as of 10:54, 12 September 2012
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Day 4Goals
Procedure
Data/ Calculations
Link to spreadsheet and chart of redone UV-Vis: Link to spreadsheet and charts of the 3 UV-Vis data on the solutions of AuNP with tris buffer. ConclusionsFor the redone AuNP/BSA UV-Vis tests done. Instead of just one peak at 550nm, there was an uncharacteristic peak at around 490-500nm as well. As a result, distilled water was also tested to see if it had been a factor in causing an adjustment to the same. In the data spreadsheet, the UV-Vis data for each of the samples then had the UV-Vis data for water subtracted from it in order to test if it was the water that had caused the peak. The graphs clearly show that without the water, the background, the second peak was removed.
|