User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/12: Difference between revisions
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==Goals== | ==Goals== | ||
* Centrifuge the 7 samples of AuNP/BSA in order to separate out the fibers | * Centrifuge the 7 samples of AuNP/BSA in order to separate out the fibers. Look to [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/11| yesterday's entry for concentrations]] | ||
*Run UV-Vis spectra's for the samples every 1 hour to test absorbency rates of the AuNP into the buffer. | *Run UV-Vis spectra's for the samples every 1 hour to test absorbency rates of the AuNP into the buffer. | ||
* begin making solutions to replicate experiment | * begin making solutions to replicate experiment. The experiment attempting to be retested is in testing the ionic strength of the refolding buffer. A [[https://blackboard.american.edu/webapps/blackboard/execute/content/file?cmd=view&content_id=_1328505_1&course_id=_49151_1| previous class]] had tested the effect of different concentrations of tris buffer on the AuNP fibers. We are attempting to replicate this and see how our results compare. | ||
==Procedure== | ==Procedure== | ||
* The samples from yesterday were centrifuged in order to separate out the fibers | * The samples from yesterday were centrifuged in order to separate out the fibers | ||
* They were then combined with 7 different concentrations of the pH 10 Tris buffer. Just as was similarly done the previous week. a serial dilution was done. | * They were then combined with 7 different concentrations of the pH 10 Tris buffer. Just as was similarly done the previous week. a serial dilution was done. | ||
*prepare tris buffer and AuNP/BSA solution at a concentration of 70 for next weeks lab. | *prepare tris buffer and AuNP/BSA solution at a concentration of 70 for next weeks lab. This stock solution will be used to run UV-Vis based on variations in tris buffer as previous students had done. | ||
==Calculations/ Data== | ==Calculations/ Data== | ||
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#Solutions went in oven at 85<sup>o</sup>C from 2:32-6:32 | #Solutions went in oven at 85<sup>o</sup>C from 2:32-6:32 | ||
* Stock solution was made by [[User:Abigail_E._Miller/Notebook/CHEM571_Lab_Project/2012/08/31| Dr. Miller]] | |||
Tris Buffer | Tris Buffer | ||
* MW= 121.14 g/mol per 1 Liter | * MW= 121.14 g/mol per 1 Liter | ||
make 100mL of solutions so..... 12.11grams of tris /100mL were mixed. | make 100mL of solutions so..... 12.11grams of tris /100mL were mixed. | ||
[[Image:AuNP in Tris buffer pH 10.png]] | |||
For further breakdown by run and concentration see attached spreadsheet | |||
Link to spreadsheet for Tris buffer pH 10 UV-Vis. | Link to spreadsheet for Tris buffer pH 10 UV-Vis. | ||
*http://openwetware.org/wiki/Image:AuNP_in_tris_buffer_pH_10.xlsx | *http://openwetware.org/wiki/Image:AuNP_in_tris_buffer_pH_10.xlsx | ||
==Conclusions== | ==Conclusions== | ||
*pH 10 AuNP fibers + Tris buffer UV-Vis data showed that: | *pH 10 AuNP fibers + Tris buffer UV-Vis data showed that: | ||
A. The results were fairly consistent with there not being an increase in the concentration of the gold nano particles. For all 7 samples, the peak stayed at generally the same wavelength for all three tests and therefore the entire length of experimentation time. However, it is important to note the drop in absorbency | A. The results were fairly consistent with there not being an increase in the concentration of the gold nano particles. For all 7 samples, the peak stayed at generally the same wavelength for all three tests and therefore the entire length of experimentation time. Thus it seems that changes in the pH of the tris buffer did not result in a noticeable change in abs. However, it is important to note the drop in absorbency for some of the concentration samples. For example, the 1x10-4 concentration shows the most obvious drop between The first time the were measured after the addition of tris (immediately) and two hours later when the third run of UV-Vis was taken. Whether or not this is simply a result of noise or actually because an increase in the amount of AuNP dissolved in the buffer solution is unknown because a blank of water was not measured during these tests. | ||
[[Image:1x10^-3 compared to 1x10^-4.png| 1x10^-3 compared to 1x10^-4]] | |||
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Revision as of 23:31, 2 October 2012
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Day 5Goals
Procedure
Calculations/ Data
Tris Buffer
make 100mL of solutions so..... 12.11grams of tris /100mL were mixed.
For further breakdown by run and concentration see attached spreadsheet Link to spreadsheet for Tris buffer pH 10 UV-Vis. Conclusions
A. The results were fairly consistent with there not being an increase in the concentration of the gold nano particles. For all 7 samples, the peak stayed at generally the same wavelength for all three tests and therefore the entire length of experimentation time. Thus it seems that changes in the pH of the tris buffer did not result in a noticeable change in abs. However, it is important to note the drop in absorbency for some of the concentration samples. For example, the 1x10-4 concentration shows the most obvious drop between The first time the were measured after the addition of tris (immediately) and two hours later when the third run of UV-Vis was taken. Whether or not this is simply a result of noise or actually because an increase in the amount of AuNP dissolved in the buffer solution is unknown because a blank of water was not measured during these tests.
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