User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/12: Difference between revisions
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==Goals== | ==Goals== | ||
* Centrifuge the 7 samples of AuNP/BSA in order to separate out the fibers | * Centrifuge the 7 samples of AuNP/BSA in order to separate out the fibers | ||
*Run UV-Vis spectra's for the samples every 1 hour to test absorbency rates of the AuNP into the buffer. | *Run UV-Vis spectra's for the samples every 1 hour to test absorbency rates of the AuNP into the buffer. | ||
* begin making solutions to replicate experiment based on article on blackboard. | |||
==Procedure== | ==Procedure== | ||
* The samples from yesterday were centrifuged in order to separate out the fibers | * The samples from yesterday were centrifuged in order to separate out the fibers | ||
* They were then combined with 7 different concentrations of the pH 10 Tris buffer. Just as was similarly done the previous week. a serial dilution was done. | * They were then combined with 7 different concentrations of the pH 10 Tris buffer. Just as was similarly done the previous week. a serial dilution was done. | ||
* | *prepare tris buffer and AuNP/BSA solution at a concentration of 70 for next weeks lab. | ||
==Calculations== | |||
==Entry title== | |||
#Solution was made with a mole ratio of 170 | |||
##2.4268M BSA solution was used | |||
###m<sub>1</sub>v<sub>1</sub>=m<sub>2</sub>v<sub>2</sub> | |||
### 24.266μM * (volume) = 1μM * 6mL | |||
### .247mL BSA solution | |||
## 2150μM HAuCl4 solution was used | |||
### 2150μM * (volume) = 6mL *(1μM*70) | |||
### .195mL HAuCl<sub><sub>4</sub></sub> solution | |||
## 6-.195mL BSA -.247mL HAuCl4 = 5.558mL H<sub>2</sub>O was added | |||
#Solutions went in oven at 85<sup>o</sup>C from 2:32-6:32 | |||
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Revision as of 11:58, 12 September 2012
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Day 5Goals
Procedure
CalculationsEntry title
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