User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/12: Difference between revisions
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* The samples from yesterday were centrifuged in order to separate out the fibers | * The samples from yesterday were centrifuged in order to separate out the fibers | ||
* They were then combined with 7 different concentrations of the pH 10 Tris buffer. Just as was similarly done the previous week. a serial dilution was done. | * They were then combined with 7 different concentrations of the pH 10 Tris buffer. Just as was similarly done the previous week. a serial dilution was done. | ||
*prepare tris buffer and AuNP/BSA solution at a concentration of 70 for next weeks lab. This stock solution will be used to run | *prepare tris buffer and AuNP/BSA solution at a concentration of 70 for next weeks lab. This stock solution will be used to run UV-Vis based on variations in tris buffer as previous students had done. | ||
==Calculations/ Data== | ==Calculations/ Data== |
Revision as of 23:29, 2 October 2012
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Day 5Goals
Procedure
Calculations/ Data
Tris Buffer
make 100mL of solutions so..... 12.11grams of tris /100mL were mixed. For further breakdown by run and concentration see attached spreadsheet Link to spreadsheet for Tris buffer pH 10 UV-Vis. Conclusions
A. The results were fairly consistent with there not being an increase in the concentration of the gold nano particles. For all 7 samples, the peak stayed at generally the same wavelength for all three tests and therefore the entire length of experimentation time. However, it is important to note the drop in absorbency for some of the concentration samples. For example, the 1x10-4 concentration shows the most obvious drop between The first time the were measured after the addition of tris (immediately) and two hours later when the third run of UV-Vis was taken. Whether or not this is simply a result of noise or actually because an increase in the amount of AuNP dissolved in the buffer solution is unknown because a blank of water was not measured during these tests.
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