User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/12

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Day 5

Goals

  • Run UV-Vis spectra's for the samples every 1 hour to test absorbency rates of the AuNP into the buffer.
  • begin making solutions to replicate experiment based on article on blackboard.
  • Abigail E. Miller 13:51, 17 September 2012 (EDT):be more specific. i have no idea what experiment and what article?

Procedure

  • The samples from yesterday were centrifuged in order to separate out the fibers
  • They were then combined with 7 different concentrations of the pH 10 Tris buffer. Just as was similarly done the previous week. a serial dilution was done.
  • prepare tris buffer and AuNP/BSA solution at a concentration of 70 for next weeks lab.

Calculations/ Data

  1. Solution was made with a mole ratio of 70
    1. 2.4268M BSA solution was used
      1. m1v1=m2v2
      2. 24.266μM * (volume) = 1μM * 6mL
      3. .247mL BSA solution
    2. 2150μM HAuCl4 solution was used
      1. 2150μM * (volume) = 6mL *(1μM*70)
      2. .195mL HAuCl4 solution
    3. 6-.195mL BSA -.247mL HAuCl4 = 5.558mL H2O was added
  2. Solutions went in oven at 85oC from 2:32-6:32


  • Abigail E. Miller 13:52, 17 September 2012 (EDT):where did the stock solutions come from? can you like to the page where they were made?

Tris Buffer

  • MW= 121.14 g/mol per 1 Liter

make 100mL of solutions so..... 12.11grams of tris /100mL were mixed.

Link to spreadsheet for Tris buffer pH 10 UV-Vis.

Conclusions

  • pH 10 AuNP fibers + Tris buffer UV-Vis data showed that:

A. The results were fairly consistent with there not being an increase in the concentration of the gold nano particles. For all 7 samples, the peak stayed at generally the same wavelength for all three tests and therefore the entire length of experimentation time. However, it is important to note the drop in absorbency in for some of the concentration samples. For example, the 1x10-4 concentration shows the most obvious drop between rounds 1 and 3 of testing. Whether or not this is simply a result of noise or actually because an increase in the amount of AuNP dissolved in the buffer solution.

  • Abigail E. Miller 13:55, 17 September 2012 (EDT):please make this note clearer. parts in italics are confusing. drop in what is observed? use time references not round one and two. what is the time after tris addition 1 hr and 24 hrs?


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