User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/18

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Procedure)
(Procedure)
Line 34: Line 34:
(note that while there were the amounts measured out- the amount of LB added to the flasks was actually less due to loss of sample in transferring it from the weigh dish to the flask)
(note that while there were the amounts measured out- the amount of LB added to the flasks was actually less due to loss of sample in transferring it from the weigh dish to the flask)
 +
 +
 +
* Next, two of the erlenmeyer flasks and two of the Fernbach flasks were placed in the [[AU_Biomaterials_Design_Lab:Protocols/Autoclave|Autoclave]] at a temperature of 121°C for 60minutes followed by a 30 minute cool down period.
 +
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 13:52, 18 September 2012

Project name Main project page
Previous entry      Next entry

Day 5

  • Protein Expressions and Starters

Goals

  • Grow E.Coli cells and have them express ADA protein.
  • Follow Protocols for making starter and expression cultures of the cells.

Procedure

(note that while there were the amounts measured out- the amount of LB added to the flasks was actually less due to loss of sample in transferring it from the weigh dish to the flask)


(note that while there were the amounts measured out- the amount of LB added to the flasks was actually less due to loss of sample in transferring it from the weigh dish to the flask)


  • Next, two of the erlenmeyer flasks and two of the Fernbach flasks were placed in the Autoclave at a temperature of 121°C for 60minutes followed by a 30 minute cool down period.



Personal tools