User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/18: Difference between revisions
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(note that while there were the amounts measured out- the amount of LB added to the flasks was actually less due to loss of sample in transferring it from the weigh dish to the flask) | (note that while there were the amounts measured out- the amount of LB added to the flasks was actually less due to loss of sample in transferring it from the weigh dish to the flask) | ||
* [[http://openwetware.org/wiki/AU_Biomaterials_Design_Lab:Protocols/Expression_Culture_Media|Expression Culture | * [[http://openwetware.org/wiki/AU_Biomaterials_Design_Lab:Protocols/Expression_Culture_Media| Expression Culture Media]] | ||
** 1L of distilled water were added to 4.8L Fernbach flasks | ** 1L of distilled water were added to 4.8L Fernbach flasks | ||
**LB was added to each flask | **LB was added to each flask |
Revision as of 22:53, 2 October 2012
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Day 5
Goals
Procedure
(note that while there were the amounts measured out- the amount of LB added to the flasks was actually less due to loss of sample in transferring it from the weigh dish to the flask)
Using the formula M1V1=M2V2 The volume or amount of kanamycin that would need to go into each of the flasks was 50 μg/mL x 35mL ÷50,000 μg = .35mL Kanamycin.
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