User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/18: Difference between revisions
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* The bacteria was inoculated by retrieving a single colony from the petri dish and dropping it into the flask. | * The bacteria was inoculated by retrieving a single colony from the petri dish and dropping it into the flask. | ||
**After all the broths | **After all the starter culture media broths cooled down, they were inoculate with transformed ''Escherichia coli''. Aseptic techniques are implemented to prevent any undesirable microbes from being included in the inoculation of the media. | ||
*''E. coli'' were transformed by the insertion of plasmid encoding for Adenosine Deaminase (ADA). | |||
* Aseptic techniques were applied by executing the inoculation process near the proximity of a Bunsen burner. The aluminum foil tops of the Erlenmeyer containing the broths were flamed in between uncapping and capping. The metal tweezers were flamed before and after use. Sterile wood sticks were used to capture a colony of the ''E.Coli'' | |||
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Revision as of 23:15, 2 October 2012
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Day 5
Goals
Procedure
(note that while there were the amounts measured out- the amount of LB added to the flasks was actually less due to loss of sample in transferring it from the weigh dish to the flask)
Using the formula M1V1=M2V2 The volume or amount of kanamycin that would need to go into each of the flasks was 50 μg/mL x 35mL ÷50,000 μg = .035mL Kanamycin.
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