User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/18: Difference between revisions
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==Procedure== | ==Procedure== | ||
--[[User:Abigail E. Miller|Abigail E. Miller]] 10:48, 7 October 2012 (EDT): note what hte starter and expression cultures are for; complete sentences...Following the protocol xxxx, we created LB broth for growing cells.... | |||
* [[http://openwetware.org/wiki/AU_Biomaterials_Design_Lab:Protocols/Starter_Culture_Media| Started Culture Protocol]] | * [[http://openwetware.org/wiki/AU_Biomaterials_Design_Lab:Protocols/Starter_Culture_Media| Started Culture Protocol]] | ||
** add 35 mL H<sub>2</sub>Oto each to 4 250mL erlenmeyer flask | ** add 35 mL H<sub>2</sub>Oto each to 4 250mL erlenmeyer flask |
Revision as of 07:48, 7 October 2012
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Day 5
Goals
Procedure--Abigail E. Miller 10:48, 7 October 2012 (EDT): note what hte starter and expression cultures are for; complete sentences...Following the protocol xxxx, we created LB broth for growing cells....
(note that while there were the amounts measured out- the amount of LB added to the flasks was actually less due to loss of sample in transferring it from the weigh dish to the flask)
Using the formula M1V1=M2V2 The volume or amount of kanamycin that would need to go into each of the flasks was 50 μg/mL x 35mL ÷50,000 μg = .035mL Kanamycin.
Preparation of Tris Buffer
the desired concentration of the stock was .1 M tris and a total of 25mL of stock was made. 1 mol/ 1L x .025 L= .025 mol .025 mol x 121.14 grams/1 mol = 3.0285 grams of tris in 25mL of water * in actuality, 3.080 grams of tris was added.
(The tris and water were not combined with the AuNP/BSa solutions today)
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