User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/18

Day 5

• Protein Expressions and Starters

Goals

• Grow E.Coli cells and have them express ADA protein.
• Follow Protocols for making starter and expression cultures of the cells.

Procedure

• Starter Culture Protocol: http://openwetware.org/wiki/AU_Biomaterials_Design_Lab:Protocols/Starter_Culture_Media
  • add 35 mL to each to 4 250mL erlenmeyer flask
  • LB was added to each flask.
    • Flask 1=.8793g
    • Flask 2=.8800g
    • Flask 3=.8748g
    • Flask 4=.8757g

(note that while there were the amounts measured out- the amount of LB added to the flasks was actually less due to loss of sample in transferring it from the weigh dish to the flask)

• Expression culture Protocols: http://openwetware.org/wiki/AU_Biomaterials_Design_Lab:Protocols/Expression_Culture_Media
  • 1L of distilled water were added to 4.8L Fernbach flasks
  • LB was added to each flask
    • Flask 1= 25.0289g
    • Flask 2= 25.0069g
    • Flask 3= 25.0020g
    • Flask 4= 25.0036g

(note that while there were the amounts measured out- the amount of LB added to the flasks was actually less due to loss of sample in transferring it from the weigh dish to the flask)