User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/19
From OpenWetWare
(Difference between revisions)
(→Entry title) |
|||
| Line 7: | Line 7: | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==Protein Expression. == | ==Protein Expression. == | ||
| - | * | + | * |
| + | |||
| + | ==Goals== | ||
| + | *Induce Protein expression. | ||
| + | * make the binding buffer and the elution buffer | ||
| + | *Continue running AuNP tests- | ||
| + | *Harvest the cells that were grown. | ||
| + | |||
| + | ==Procedure== | ||
| + | *Protein Expression | ||
| + | #This morning at 8 am, the starter cultures were centrifuged at 4500 rpm for 15 minutes and then resuspended the cell pellets in 4mL of fresh LB. | ||
| + | # Inoculate the [[AU_Biomaterials_Design_Lab:Protocols/Expression_Culture_Media|expression culture media]] by dividing the resuspended cells among the Fernbach flasks. | ||
| + | # Incubate the expression cultures at 37C and 160rpm until the absorbance of the media at 600nm = 0.6. | ||
| + | # Add 1mL of 0.4M [[AU_Biomaterials_Design_Lab:Materials/IPTG|IPTG]] to each flask in order to induce protein expression | ||
| + | # Continue shaking for 3-4 hours | ||
| + | # Harvest the cells by centrifuging at 4500rpm for 15 minutes | ||
| + | |||
| + | |||
| + | |||
| + | ==Conclusions== | ||
| + | *sample 1 looked a little different when the samples were looked at this morning prior to centrifuging. It was a different shade of yellow in the erlenmeyer flask. | ||
Revision as of 15:26, 19 September 2012
 Project name
|  Main project page Previous entry Next entry
|
Protein Expression.Goals
Procedure
Conclusions
| |
