User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/19: Difference between revisions
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# Harvest the cells by centrifuging at 4500rpm for 15 minutes | # Harvest the cells by centrifuging at 4500rpm for 15 minutes | ||
* Binding Buffer: 1 liter total solution at pH 7.4 | |||
Theoretical calculations | |||
#20mM Tris= .020M/L Tris x1 L= .020 mols x 121.14 g/mol = 2.4228 g Tris. | |||
** similar calculations were done for all compounds added into the buffer solution. | |||
# .5M NaCl= 29.2195 g NaCl | |||
# 30mM Imidazole | |||
==Conclusions== | ==Conclusions== |
Revision as of 12:31, 19 September 2012
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Protein Expression.Goals
Procedure
Theoretical calculations
Conclusions
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