User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/19: Difference between revisions
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* Refer to [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/09/19| Mary's Notebook]] for the actual amounts of buffer components measured out and added to solution. | * Refer to [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/09/19| Mary's Notebook]] for the actual amounts of buffer components measured out and added to solution. | ||
--[[User:Abigail E. Miller|Abigail E. Miller]] 10:54, 7 October 2012 (EDT)did you have to adjust the pH? | |||
==Conclusions== | ==Conclusions== |
Revision as of 07:54, 7 October 2012
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Protein Expression.Goals
Procedure
--Abigail E. Miller 10:53, 7 October 2012 (EDT)it is not clear that this was done in the morning.
--Abigail E. Miller 10:53, 7 October 2012 (EDT)this is too much like the protocol. what did you actually do? at what time was the IPTG added? you did not measure the OD at 600nm. what time did you harvest the cells? therefore how long were they allowed to express? how many times did the cells have to be spun down? what deviations did you make from the protocol - I know you separated flask 1 form the other three. that is not clear here. Calculations
a. Theoretical calculations
a. Theoretical calculations
--Abigail E. Miller 10:54, 7 October 2012 (EDT)did you have to adjust the pH? Conclusions
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