User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/25: Difference between revisions
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[[Image:Tris2trialsvariousmolar.JPG| Tris varying concentrations at pH 10]] | [[Image:Tris2trialsvariousmolar.JPG| Tris varying concentrations at pH 10]] | ||
link for excel [[Image:TrisvaryingmolarUVVis.xlsx| Spreadsheets]] | |||
==Conclusions== | ==Conclusions== |
Revision as of 23:51, 2 October 2012
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Separate ProteinsGoals
Procedure
DATA/Calculations
to make a 25mL stock solution of the tris buffer at a pH of 10 1mol/L x .025= .025 mol .025 mol x 121.14 g/mol = 3.0285 g of tris To get the necessary concentrations of tris into the gold/BSA solutions, the M1V1=M2V2 formula was used to calculate the volume of tris needed. From there, the value of the volume of tris was subtracted from 1 in order to determine the amount of water that would be added to make the desired concentration.
link for excel File:TrisvaryingmolarUVVis.xlsx Conclusions
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