User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/25: Difference between revisions
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#Meanwhile, filter the binding buffer and elution buffer in order to purify them using a vacuum system of filtration and filter paper with a membrane filter in which the holes measure 450nm. | #Meanwhile, filter the binding buffer and elution buffer in order to purify them using a vacuum system of filtration and filter paper with a membrane filter in which the holes measure 450nm. | ||
#Once the centrifuging is completed. Use the same vacuum filter system to separate the proteins from everything else in the vials (organelles, buffer, etc.) | #Once the centrifuging is completed. Use the same vacuum filter system to separate the proteins from everything else in the vials (organelles, buffer, etc.) | ||
# The protein was collected in falcon vials and refrigerated. | |||
*UV-Vis | *UV-Vis |
Revision as of 02:02, 26 September 2012
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Separate ProteinsGoals
Procedure
DATA/Calculations
to make a 25mL stock solution of the tris buffer at a pH of 10 1mol/L x .025= .025 mol .025 mol x 121.14 g/mol = 3.0285 g of tris To get the necessary concentrations of tris into the gold/BSA solutions, the M1V1=M2V2 formula was used to calculate the volume of tris needed. From there, the value of the volume of tris was subtracted from 1 in order to determine the amount of water that would be added to make the desired concentration. Conclusions
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