User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/02: Difference between revisions
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# 1mg of HRP was dissolved in 1mL of distilled water. | # 1mg of HRP was dissolved in 1mL of distilled water. | ||
Based on the other groups tests, we decided to keep all our variables constant except for the percent by volume of the AuNP added into each cuvet. We decided that between the two separate trials, we would vary the concentration of the AAP because on the other groups results, it seems that the concentrations 156.25 mM and 312 mM were the most linear. | Based on the other groups tests, we decided to keep all our variables constant except for the percent by volume of the AuNP added into each cuvet.The Gold Nanoparticle were made in a 1% citrate solution rather than in BSa. We decided that between the two separate trials, we would vary the concentration of the AAP because on the other groups results, it seems that the concentrations 156.25 mM and 312 mM were the most linear. | ||
Because of changes in calculations and incorrect data results from the UV-Vis instrument, we were unable to run any of our absorbances today. | Because of changes in calculations and incorrect data results from the UV-Vis instrument, we were unable to run any of our absorbances today. |
Revision as of 21:59, 4 October 2012
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Horseradish Peroxidase Assay AbsorbanceGoals
Procedure
distilled water. It was then further diluted by taking 1mL of the solution in 50mL of 0.2 M sodium phosphate buffer.
Based on the other groups tests, we decided to keep all our variables constant except for the percent by volume of the AuNP added into each cuvet.The Gold Nanoparticle were made in a 1% citrate solution rather than in BSa. We decided that between the two separate trials, we would vary the concentration of the AAP because on the other groups results, it seems that the concentrations 156.25 mM and 312 mM were the most linear. Because of changes in calculations and incorrect data results from the UV-Vis instrument, we were unable to run any of our absorbances today. Calculations and Data |