User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/03

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Kinetics

Goals

Test activity of HRP int he presence of AuNP by using a Uv-Vis Absorbance HRP assay.

Procedure

We ran multiple UV-Vis spectras of the HRP with the gold nanoparticles to examine the impact that the NPs had on the kinetics of the reaction. We ran each run for 10 minutes and collected the various spectras. We maintained the same concentration in the cuvet of all the reagents except for the concentration of the gold nanoparticles. As a result, we were examining the effect that gold nanoparticles had on the rate of the reaction.

Data

We ran 5 main tests and several of the tests multiple times to confirm the data. The multiple tests showed that the experiment was not really reproducible because we seemed to be getting different spectras every time.

Trial run A	'	'	'	'	'	'
Cuvet	hydrogen peroxide concentration (mM)	Iodophenol in DMSO concentration (μM)	AAP Concentration (μM)	HRP concentration (μM)	μL of gold np added	μL of water added
1( standard)	1.7	18	156.25	2.3	0	630
1	1.7	18	156.25	2.3	20	610
2	1.7	18	156.25	2.3	40	590
3	1.7	18	156.25	2.3	60	570
4	1.7	18	156.25	2.3	80	550
5	1.7	18	156.25	2.3	100	530
Total volume added	750 μL	10 μL	35 μL	22 μL

The amount of HRP was changed from 22μL to 33μL because the 22μL in the cuvet did not give a linear spectra, just a horizontal line. More was needed in order to really push the reaction.