User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/09: Difference between revisions
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==Procedure== | ==Procedure== | ||
* The AA was calibrated using standard solutions made by [[User:Abigail E. Miller/Notebook/CHEM571 Lab Project/2012/10/08| Dr. Miller]]. The concentrations of these standard solutions were 5,8,10,15,20,25,30 and 40 ppm. The | * The AA was calibrated using standard solutions made by [[User:Abigail E. Miller/Notebook/CHEM571 Lab Project/2012/10/08| Dr. Miller]]. The concentrations of these standard solutions were 5,8,10,15,20,25,30 and 40 ppm. The AA runs were blanked with 1M HCl. | ||
*Deionized water was run through the AA between samples. | *Deionized water was run through the AA between samples. | ||
* The Au/BSA samples had been made on [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26| 2012/09/26]]. | * The Au/BSA samples had been made on [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26| 2012/09/26]]. | ||
*The samples were runt through the AA and blanked with 1M HCl between every 4 samples. | **Fluid was removed from fibers before being analyzed, as fibers could clog the tubing. | ||
*The samples were runt through the AA and blanked with 1M HCl between every 4 samples. | |||
==Data/Results== | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Standard''' | |||
| align="center" style="background:#f0f0f0;"|'''True Value Concentration''' | |||
| align="center" style="background:#f0f0f0;"|'''Absorbance''' | |||
|- | |||
| 5||-||0.0913 | |||
|- | |||
| 8||-||0.1547 | |||
|- | |||
| 10||-||0.1993 | |||
|- | |||
| 15||-||0.3175 | |||
|- | |||
| 20||-||0.4059 | |||
|- | |||
| 25||-||0.4918 | |||
|- | |||
| 30||-||0.5928 | |||
|- | |||
| 40||-||0.7299 | |||
|- | |||
| Blank||-||0.0198 | |||
|} | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Sample''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration''' | |||
| align="center" style="background:#f0f0f0;"|'''Absorbance''' | |||
|- | |||
| 60||11.0972||0.2228 | |||
|- | |||
| 80||15.3973||0.3028 | |||
|- | |||
| 100||21.6271||0.4187 | |||
|- | |||
| 120||21.7669||0.4213 | |||
|- | |||
| 128||22.4442||0.4339 | |||
|- | |||
| 130||23.7718||0.4586 | |||
|- | |||
| 132||21.3584||0.4137 | |||
|- | |||
| 133||23.0515||0.4452 | |||
|- | |||
| 134||19.1492||0.3726 | |||
|- | |||
| 136||25.3629||0.4882 | |||
|- | |||
| 138||24.6318||0.4746 | |||
|- | |||
| 140||24.2126||0.4668 | |||
|- | |||
| 160||27.2603||0.5235 | |||
|- | |||
| 170||29.3405||0.5622 | |||
|} | |||
==Conclusions | |||
* Absorbance and concentrations of standards and solutions. It can be seen that there is a general trend. as concentration increases, so does absorbance. There is a drop and then a spike and the 138-140 range. | |||
[[Image:AASRound1.png]] | |||
[[Image:AA calibration cuve.png| AA calibration curves]] | |||
==Conclusions== | |||
*For unknown reasons, there were no fibers present in the solution. But it was apparent that the gold had reacted because the solution was purple. Therefore, we went ahead with the experiment with the intent or repeating it at a later date. | *For unknown reasons, there were no fibers present in the solution. But it was apparent that the gold had reacted because the solution was purple. Therefore, we went ahead with the experiment with the intent or repeating it at a later date. | ||
* The AAS allowed us to conclude that the measured concentration of gold in the solution was different from what we had anticipated when we made the solutions at specific mole ratios. This could indicate the fact that the lack of NP fibers in these samples resulted in the variation in concentration observed. | |||
Revision as of 22:59, 6 December 2012
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Atomic Absorption SpectrumGoals
Procedure
Data/Results
Conclusions
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