User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/16: Difference between revisions
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* a new stock of luminol solution was made at a pH of 10.56. For full details on preparation, calculation, and concentration please see [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/16| Mary's Notebook]] | * a new stock of luminol solution was made at a pH of 10.56. For full details on preparation, calculation, and concentration please see [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/16| Mary's Notebook]] | ||
* The other reagents were either left over from the HRP absorbance assay from [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/02| another week]] or were remade in using the same method. | * The other reagents were either left over from the HRP absorbance assay from [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/02| another week]] or were remade in using the same method. | ||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Reagent''' | |||
| align="center" style="background:#f0f0f0;"|'''Desired concentration in cuvet''' | |||
| align="center" style="background:#f0f0f0;"|'''M1''' | |||
| align="center" style="background:#f0f0f0;"|'''V1''' | |||
| align="center" style="background:#f0f0f0;"|'''M2''' | |||
| align="center" style="background:#f0f0f0;"|'''V2''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''HRP''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
|- | |||
| Iodophenol||.18mM||10mM||27μL||.18mM||1500μL||||Concentration in Cuvet||Volume | |||
|- | |||
| H202||.1mM||1.7mM||88μL||.1mM||1500μL||||20mM||30μL | |||
|- | |||
| Luminol||3mM||30mM||450μL||3mM||1500μL||||22mM||33μL | |||
|- | |||
| ||||||||||||||24mM||36μL | |||
|- | |||
| ||||||||||||||26mM||39μL | |||
|- | |||
| ||||||||||||||28mM||42μL | |||
|} | |||
==Calculations== | ==Calculations== |
Revision as of 18:25, 23 October 2012
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PCR of ADA MutationsGoals
Preparation
Concentration of plasmid needed for experiment= 100ng/μL
.56mg= 5600 ng M1V1= M2V2 5600 ng/μL x V1= 100ng x 1000μL
V2= 17.857μL is the amount of plasmid solution pipetted out in order to get a concentration of 100ng/μL Procedure
Calculations
Total Volume in cuvet= 1500μL Conclusions
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