User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/16: Difference between revisions
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.56mg= 5600 ng | .56mg= 5600 ng | ||
M<sub>1</sub>V<sub>1</sub>= M<sub>2</sub>V<sub>2</sub> | M<sub>1</sub>V<sub>1</sub>= M<sub>2</sub>V<sub>2</sub> | ||
5600 ng/μL x V<sub>1</sub>= 100ng x 1000μL | 5600 ng/μL x V<sub>1</sub>= 100ng x 1000μL | ||
*(The container containing the plasmid only had room for 1mL of water to be added- as a result, a dilution needed to be done in order to get the appropriate concentration. | *(The container containing the plasmid only had room for 1mL of water to be added- as a result, a dilution needed to be done in order to get the appropriate concentration. 1mL of water was added to dissolve the plasmid prior to the dilution | ||
V<sub>2</sub>= 17.857μL is the amount of plasmid solution pipetted out in order to get a concentration of 100ng/μL | V<sub>2</sub>= 17.857μL is the amount of plasmid solution pipetted out in order to get a concentration of 100ng/μL | ||
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* a new stock of luminol solution was made at a pH of 10.56. For full details on preparation, calculation, and concentration please see [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/16| Mary's Notebook]] | * a new stock of luminol solution was made at a pH of 10.56. For full details on preparation, calculation, and concentration please see [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/16| Mary's Notebook]] | ||
* The other reagents were either left over from the HRP absorbance assay from [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/02| another week]] or were remade in using the same method. | * The other reagents were either left over from the HRP absorbance assay from [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/02| another week]] or were remade in using the same method. The final concentrations and volumes are as follows. | ||
{| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|'''Reagent''' | | align="center" style="background:#f0f0f0;"|'''Reagent''' | ||
| align="center" style="background:#f0f0f0;"|'''Desired concentration in | | align="center" style="background:#f0f0f0;"|'''Desired concentration in cuvette''' | ||
| align="center" style="background:#f0f0f0;"|'''M1''' | | align="center" style="background:#f0f0f0;"|'''M1''' | ||
| align="center" style="background:#f0f0f0;"|'''V1''' | | align="center" style="background:#f0f0f0;"|'''V1''' | ||
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| align="center" style="background:#f0f0f0;"|'''''' | | align="center" style="background:#f0f0f0;"|'''''' | ||
|- | |- | ||
| Iodophenol||.18mM||10mM||27μL||.18mM||1500μL||||Concentration in | | Iodophenol||.18mM||10mM||27μL||.18mM||1500μL||||Concentration of compound in Cuvette||Volume | ||
|- | |- | ||
| H202||.1mM||1.7mM||88μL||.1mM||1500μL||||20mM||30μL | | H202||.1mM||1.7mM||88μL||.1mM||1500μL||||20mM||30μL |
Revision as of 16:43, 21 November 2012
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PCR of ADA MutationsGoals
Preparation
Concentration of plasmid needed for experiment= 100ng/μL
.56mg= 5600 ng M1V1= M2V2 5600 ng/μL x V1= 100ng x 1000μL
V2= 17.857μL is the amount of plasmid solution pipetted out in order to get a concentration of 100ng/μL Procedure
Calculations
Total Volume in cuvet= 1500μL Conclusions
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