User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/23

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(Procedure)
(Procedure)
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* For the Starter culture media and expression culture media, the same procedure from [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/18| September 18]]
* For the Starter culture media and expression culture media, the same procedure from [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/18| September 18]]
* The samples of broth were made and autoclaved today. The antibiotic and the E.coli colony were also added into the starter culture media.
* The samples of broth were made and autoclaved today. The antibiotic and the E.coli colony were also added into the starter culture media.
 +
#The LB was weighed out into 4 large Fernbach flasks and 4 small erlyenmeyer flasks.
* For AAS: The 14 samples produced fibers this time and as a result, the fibers needed to be removed which was done through centrifuging and then pipetting out the solution without the fibers.  For everything done in regards to the AAS today please see either Michael's or [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/23| Mary's notebook.]]
* For AAS: The 14 samples produced fibers this time and as a result, the fibers needed to be removed which was done through centrifuging and then pipetting out the solution without the fibers.  For everything done in regards to the AAS today please see either Michael's or [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/23| Mary's notebook.]]

Revision as of 18:53, 21 November 2012

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More ADA and AAS

Goals

  • Run AAS from Au/BSA solutions
  • Make starter culture media and expression culture media in order to produce more ADA protein.

Procedure

  • For the Starter culture media and expression culture media, the same procedure from September 18
  • The samples of broth were made and autoclaved today. The antibiotic and the E.coli colony were also added into the starter culture media.
  1. The LB was weighed out into 4 large Fernbach flasks and 4 small erlyenmeyer flasks.
  • For AAS: The 14 samples produced fibers this time and as a result, the fibers needed to be removed which was done through centrifuging and then pipetting out the solution without the fibers. For everything done in regards to the AAS today please see either Michael's or Mary's notebook.

Data

Starter Culture Media-- amount of LB added to each flask

  1. Flask 1: 25.0095g
  2. Flask 2: 25.0059g
  3. Flask 3: 25.009g
  4. Flask 4: 25.0008g

Expression Culture Media:

  1. Flask 1: .8744g
  2. Flask 2: .8775g
  3. Flask 3: .8745g
  4. Flask 4L .8749g
  • AA DATA
Standard Concentration Absorbance Background
5-0.1132-0.0057
8-0.1835-0.001
10-0.22050.0008
15-0.3209-0.0018
20-0.42880.0033
25-0.52790.0043
30-0.59890.0062
40-0.77580.0052
Blank-0.01340.009


Sample Concentration Absorbance Background
6010.22470.22740.0055
8016.15690.33990.0071
100-1.11250.0124-0.0008
12021.2560.43660.0073
128-1.24960.00980.0002
130-0.88580.01670.0029
132-1.39730.0070.0036
133-1.20740.01060.0034
134-1.30240.00880.0014
136-1.44470.00610.003
138-1.10730.0125-0.0002
140-1.23380.01010.0028
160-1.03870.01380.002
170-0.09690.01630.002


AA Standard from 10/23

Calculations of comparisons between measured gold concentration and actual concentration after the removal of the fibers from the solutions to come in the future.

Image:AAS data.xlsx


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