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Transformation, protein expression, lysozyme Uv-Vis
Goals
- Transform PCR product into E.Coli Cells
- Induce ADA protein expression
- Run Uv-Vis of the lysozyme and AuNP solution
Procedure
- The PCR product was transformed by following the Transformation Protocol
- ADA protein was expressed in the same manner as on 2012/09/19
- 5mL of .4M IPTG solution was made and 1mL was placed into each of the 4 fernbach flasks.
- .005L x.4M= .002 moles x 238.31g/mol= .477g IPTG in 5mL. Actual= .4754g in 5mL of water.
- For information regarding the Lysozyme please see either Mikes notebook or Marys notebook. The lysozyme did create purple gold nanoparticle fibers upon removal from the oven.
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