User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/07: Difference between revisions
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==Procedure== | ==Procedure== | ||
* A stock solution of 1mg/mL BSA was prepared | * A stock solution of 1mg/mL BSA was prepared (actual concentration = 1.02mg/mL). 10.2mg BSA added to 10mL of water in a volumetric flask. | ||
*In order to determine the concentration of the ADA collected in the fracs, standard solutions had to be made and run through the UV-Vis first. Seven standard solutions were prepared with 1X Bradford Reagent, varying amounts of BSA stock, and autoclaved water. These solutions were analyzed in the UV-Vis from 500-800 nm. | *In order to determine the concentration of the ADA collected in the fracs, standard solutions had to be made and run through the UV-Vis first. Seven standard solutions were prepared with 1X Bradford Reagent, varying amounts of BSA stock, and autoclaved water. These solutions were analyzed in the UV-Vis from 500-800 nm. | ||
Revision as of 12:37, 24 November 2012
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Bradford AssayGoals
Procedure
DATA
Concentrations, absorbances, for the collected fracs of ADA
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