User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/07: Difference between revisions
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* The absorbance values at 595 nm of each solution were graphed versus BSA concentration in mg/mL to produce a calibration curve. | * The absorbance values at 595 nm of each solution were graphed versus BSA concentration in mg/mL to produce a calibration curve. | ||
==DATA== | |||
[[Image:Bradford_assay.png]] | [[Image:Bradford_assay.png]] | ||
* | * Linear regression was used to determine the equation of the line | ||
* When these samples were originally run, they produced absorbances over 1 which resulted in us needing to dilute the solution further. As a result, all solutions were diluted by a half to 715μL of original solution with an additional 715μL of water. | |||
==DATA== | ==DATA== |
Revision as of 14:41, 11 November 2012
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Bradford AssayGoals
Procedure
DATA
DATA |