User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/07: Difference between revisions
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* After the calibration curve was made for the standards, the concentration of the ADA collected on the two days were analyzed. We ran both fracs that possessed the majority of the sample from [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26| 2012/09/26]] and only frac number 2 from [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/06| 2012/11/06]] | * After the calibration curve was made for the standards, the concentration of the ADA collected on the two days were analyzed. We ran both fracs that possessed the majority of the sample from [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26| 2012/09/26]] and only frac number 2 from [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/06| 2012/11/06]] | ||
* In a cuvet, 7.5μL of autclaved water, 7.5 μL of a fraction and 715μL 1X bradford Reagent were added. In total 3 cuvet samples were run through the UV-Vis. | |||
**Because the spectra for frac #8 and Frac New had an absorbance of over 1, those cuvet samples were diluted in half using the same procedure as for the standard solutions in order to get an acceptable (below 1) absorbance) | |||
==DATA== | ==DATA== |
Revision as of 15:09, 11 November 2012
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Bradford AssayGoals
Procedure
DATA
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