User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/13: Difference between revisions
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**M<sub>1</sub>V<sub>1</sub>=M<sub>2</sub>V<sub>2</sub> | **M<sub>1</sub>V<sub>1</sub>=M<sub>2</sub>V<sub>2</sub> | ||
65μM * 3μL= M<sub>2</sub> * 1000μL | 65μM * 3μL= M<sub>2</sub> * 1000μL | ||
dilution 1= .195μM | **dilution 1= .195μM | ||
dilution 2= 97.5nM | **dilution 2= 97.5nM | ||
dilution3= 48.7nM | **dilution3= 48.7nM | ||
dilute 4= 24.38nM | **dilute 4= 24.38nM | ||
Revision as of 12:34, 15 November 2012
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ADA Kinetics and moreGoals
Procedure
.05 x .05= .0025 moles .0025 moles x 268.07g/1mol = .6702g sodium phosphate in 50 mL H20. Actual amount = .6705. pH of 7.4 was achieve by adding roughly 3 drops of 12M HCl from a glass pasture pipette.
(0.00373 M)(V1)=(5 mL)(0.1×10-3 M) V1 = 0.134 mL
65μM * 3μL= M2 * 1000μL
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