User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/11/13: Difference between revisions
From OpenWetWare
(fix raw html notebook nav) |
|||
(3 intermediate revisions by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
Line 14: | Line 14: | ||
==Procedure== | ==Procedure== | ||
* For procedure and data regarding the UV-Vis and the AA of the HRP and gold nanoparticle solutions, please see either [[User:Melissa Novy/Notebook/CHEM-571/2012/11/13| Melissa's notebook]] or [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/11/13| Dhea's notebook.]] | * For procedure and data regarding the UV-Vis and the AA of the HRP and gold nanoparticle solutions, please see either [[User:Melissa Novy/Notebook/CHEM-571/2012/11/13| Melissa's notebook]] or [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/11/13| Dhea's notebook.]] | ||
* The Adenosine Deaminase Kinetic assay was done by following the Assay [[AU Biomaterials Design Lab:Protocols/ADA Activity Assay| Protocol]] | * The Adenosine Deaminase Kinetic assay was done by following the Assay [[AU Biomaterials Design Lab:Protocols/ADA Activity Assay| Protocol]] | ||
Line 35: | Line 36: | ||
**dilute 4= 24.38nM | **dilute 4= 24.38nM | ||
==Data== | |||
*Graphs of the Spectras | |||
[[Image:Adacharts.png]] | |||
Latest revision as of 22:15, 26 September 2017
Project name | Main project page Previous entry Next entry |
ADA Kinetics and moreGoals
Procedure
.05 x .05= .0025 moles .0025 moles x 268.07g/1mol = .6702g sodium phosphate in 50 mL H20. Actual amount = .6705. pH of 7.4 was achieve by adding roughly 3 drops of 12M HCl from a glass pasture pipette.
(0.00373 M)(V1)=(5 mL)(0.1×10-3 M) V1 = 0.134 mL
65μM * 3μL= M2 * 1000μL
Data
|