User:Puja Mody/Notebook/Magnetite Shenanigans/2014/09/22: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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==Entry title==
==UV-Vis standards==
* Insert content here...
* Standards needed to be made of R6G in order to calculate the amount of dye that was absorbed and how much dye remains in the solution.  
 
Molar Absorptivity of R6G: 116000 LMol<sup>-1</sup>cm<sup>-1</sup>
Assumed stock concentration: .005 M
 
*Because the absorbance of R6G is so high, standard concentrations needed to be diluted to below 8 μM.
 
Table 1. Standard concentrations in 2mL solutions
 
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Standard Concentrations (uM)'''
| align="center" style="background:#f0f0f0;"|'''Actual Concentration (M)'''
| align="center" style="background:#f0f0f0;"|'''Absorbance'''
|-
| 0||0||0
|-
| 2||1.59483E-06||0.185
|-
| 4||3.18966E-06||0.37
|-
| 6||4.78448E-06||0.555
|-
| 8||6.37931E-06||0.74
|}
 
[[Image:R6G calibration curve.jpg]]
 
*The concentration of the original stock solution was 398.7μM
 
Table 2: Actual concentration of dye in eppendorf samples
 
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Sample number'''
| align="center" style="background:#f0f0f0;"|'''believed concentration'''
| align="center" style="background:#f0f0f0;"|'''Concentration in 1mL solution (uM)'''
| align="center" style="background:#f0f0f0;"|'''molar concentration in epp tubes (M)'''
|-
| 1||20||15.94827586||1.59483E-05
|-
| 2||40||31.89655172||3.18966E-05
|-
| 3||60||47.84482759||4.78448E-05
|-
| 4||80||63.79310345||6.37931E-05
|-
| 5||100||79.74137931||7.97414E-05
|-
| 6||150||119.612069||0.000119612
|-
| 7||200||159.4827586||0.000159483
|}
 
==Notes==
-These samples were left for too long without being tested and resulted in some of the dye releasing out of the complexes. For this reason, UV-Vis of these samples were not fully run and new samples were made.  




Latest revision as of 00:20, 27 September 2017

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UV-Vis standards

  • Standards needed to be made of R6G in order to calculate the amount of dye that was absorbed and how much dye remains in the solution.

Molar Absorptivity of R6G: 116000 LMol-1cm-1 Assumed stock concentration: .005 M

  • Because the absorbance of R6G is so high, standard concentrations needed to be diluted to below 8 μM.

Table 1. Standard concentrations in 2mL solutions

Standard Concentrations (uM) Actual Concentration (M) Absorbance
0 0 0
2 1.59483E-06 0.185
4 3.18966E-06 0.37
6 4.78448E-06 0.555
8 6.37931E-06 0.74

  • The concentration of the original stock solution was 398.7μM

Table 2: Actual concentration of dye in eppendorf samples

Sample number believed concentration Concentration in 1mL solution (uM) molar concentration in epp tubes (M)
1 20 15.94827586 1.59483E-05
2 40 31.89655172 3.18966E-05
3 60 47.84482759 4.78448E-05
4 80 63.79310345 6.37931E-05
5 100 79.74137931 7.97414E-05
6 150 119.612069 0.000119612
7 200 159.4827586 0.000159483

Notes

-These samples were left for too long without being tested and resulted in some of the dye releasing out of the complexes. For this reason, UV-Vis of these samples were not fully run and new samples were made.



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