User:Puja Mody/Notebook/Magnetite Shenanigans/2014/09/24: Difference between revisions
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== | ==Magnetic Stimulus Testing== | ||
General Protocol: | |||
# Centrifuge the 1.5 mL eppendorf tubes with the samples for 15 minutes at 13000 rpm | |||
# Remove the R6G supernatant and store in clean eppendorf tub | |||
# Add 1 mL deionized H<sub>2</sub>O to the eppendorf tube. | |||
# Remove the deionized water. Repeat this rinsing process a minimum of three times | |||
# Add 1.3 mL of Water to the eppendorf tube | |||
# Place samples on UV/Fluorescence light to see if dye is present in hydrogel samples | |||
#Centrifuge the samples in the same manner as in step 1. | |||
# Remove this deionized water and place in a clean eppendorf tube for later testing ( in order to test if dye is coming out with centrifuging) | |||
# Add 1.5 mL of water to sample | |||
#Vortex the sample for ~2 minutes (until the sample is in homogeneous suspension) | |||
# Apply magnet to the outside of the eppendorf tube for a minimum of 30 seconds. (until all the sample has aggregated | |||
#Vortex sample again to re-suspend the hydrogels (at least 15 seconds) | |||
#Re-apply magnet | |||
#Complete this vortex/magnet process for 4 minutes | |||
#Analyze the water using fluorescence | |||
==Analysis of the supernatant== | |||
#Place the supernatant in a clean cuvet and place into UV-Vis. Run UV-Vis analysis for 200-800nm. | |||
*If absorbance is too high, dilute samples in order to determine concentration of R6G remaining. | |||
Revision as of 16:19, 23 September 2014
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Magnetic Stimulus TestingGeneral Protocol:
Analysis of the supernatant
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