User:Radoslaw Kwasniak/Notebook/Transcription factor delivery through T3SS/2012/07/11: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 7: Line 7:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Overnight growth check==
==Overnight growth check==
* BBA_B0030 (good - turbid)
* BBa_B0030 (good - turbid)
* BBA_K11309 (good - turbid)
[[* BBa_K13009 (good - turbid)|http://partsregistry.org/Part:BBa_K113009]]


==Miniprep==
==Miniprep==

Revision as of 11:03, 12 July 2012

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>

Overnight growth check

  • BBa_B0030 (good - turbid)

http://partsregistry.org/Part:BBa_K113009

Miniprep

Used QIA Gen

  • BBA_B0030 => 30μl DNA
  • BBA_K11309 => 30μl DNA

PCR - test

Checked PCR kit (Invitrogen using katE primers and Mastermix (Rhyan))

Reaction

Conditions

1% Agarose Gel

Prepared: 0.65g of Agarose with 65ml of 1xTAE Loading

  1. 1KB NEB Ladder - 10μL
  2. BBa_B0030 (RBS - undigested) - 5μL
  3. BBa_K113009 (PROMOTER ARA - undigested) - 5μL
  4. BLANK
  5. PCR katE, MASTER MIX (Ryhan)- 10μL
  6. BLANK
  7. PCR katE, Invitrogen - 50μL
  8. PCR katE, Invitrogen - 5μL



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Radoslaw_Kwasniak/Notebook/Transcription_factor_delivery_through_T3SS/2012/07/11&oldid=613866"