User:Radoslaw Kwasniak/Notebook/Transcription factor delivery through T3SS/2012/07/11: Difference between revisions

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* BBA_K11309 => 30μl DNA
* BBA_K11309 => 30μl DNA


μ==PCR - test==
==PCR - test==
Checked PCR kit (Invitrogen using katE primers and Mastermix (Rhyan))
Checked PCR kit (Invitrogen using katE primers and Mastermix (Rhyan))


Revision as of 11:01, 12 July 2012

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Overnight growth check

  • BBA_B0030 (good - turbid)
  • BBA_K11309 (good - turbid)

Miniprep

Used QIA Gen

  • BBA_B0030 => 30μl DNA
  • BBA_K11309 => 30μl DNA

PCR - test

Checked PCR kit (Invitrogen using katE primers and Mastermix (Rhyan))

Reaction

Conditions

1% Agarose Gel

Prepared: 0.65g of Agarose with 65ml of 1xTAE Loading

  1. 1KB NEB Ladder - 10μL
  2. BBa_B0030 (RBS - undigested) - 5μL
  3. BBa_K113009 (PROMOTER ARA - undigested) - 5μL
  4. BLANK
  5. PCR katE, MASTER MIX (Ryhan)- 10μL
  6. BLANK
  7. PCR katE, Invitrogen - 50μL
  8. PCR katE, Invitrogen - 5μL



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