User:Radoslaw Kwasniak/Notebook/Transcription factor delivery through T3SS/2012/07/11

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(Overnight growth check)
(Miniprep)
Line 16: Line 16:
* BBA_K11309 => 30μl DNA
* BBA_K11309 => 30μl DNA
 +
==PCR - test==
 +
Checked PCR kit (Invitrogen using katE primers and Mastermix (Rhyan))
 +
Reaction
 +
*
 +
Conditions
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Revision as of 13:53, 12 July 2012

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Overnight growth check

  • BBA_B0030 (good - turbid)
  • BBA_K11309 (good - turbid)

Miniprep

Used QIA Gen

  • BBA_B0030 => 30μl DNA
  • BBA_K11309 => 30μl DNA

PCR - test

Checked PCR kit (Invitrogen using katE primers and Mastermix (Rhyan))

Reaction

Conditions


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