User:Rebeca Rodriguez/Notebook/Chem 471/2015/11/11: Difference between revisions
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==Data== | ==Data== | ||
The spectrum for the overall blank with no protease or fiber sample was subtracted from all other spectra (blank and sample spectra). The spectra were further corrected by subtracting the absorbance at the isosbestic point. This data was redone from November 4th, because of the variation. | The spectrum for the overall blank with no protease or fiber sample was subtracted from all other spectra (blank and sample spectra). The spectra were further corrected by subtracting the absorbance at the isosbestic point. This data was redone from November 4th, because of the variation. These data points did not reflect any change. This was going to be replicated again, but there was no more fluorescence reagent. | ||
[[Image:Bradford_10nM_Spectra_Nov_14.png]] | [[Image:Bradford_10nM_Spectra_Nov_14.png]] | ||
[[Image:Bradford_Abs_v_Time_Nov_14_.png]] | |||
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Revision as of 16:34, 23 November 2015
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Repeat of Bradford for 10 nm Proteinase KRepeat Bradford analysis of Degradation of AuNP Fibers using 10 nm Proteinase K.
Bradford Analysis of Protease DegradationThe general procedure today was taken from Dr. Hartings's notebook. All samples will be incubated in eppendorf tubes, which will be placed in the 37°C water bath on the shaker. Samples should all contain the same concentration of materials. You should make samples for measurements at 10 minutes, 15 minutes, 30 minutes, 45 minutes, 1 hr, 1.5 hr, 2 hr. (Note, this is for the first time you do this. As you get better, I will expect you to get more measurements done).
DataThe spectrum for the overall blank with no protease or fiber sample was subtracted from all other spectra (blank and sample spectra). The spectra were further corrected by subtracting the absorbance at the isosbestic point. This data was redone from November 4th, because of the variation. These data points did not reflect any change. This was going to be replicated again, but there was no more fluorescence reagent. |