User:Robert J. Schiemann/Notebook/Lipsick Lab/2010/04/09

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Spermatogenesis

  • Continue collecting virgins for stable control line.
  • Continued dissections
    • Wash 3 x 15 minutes in 1x PBST
    • Incubate with 1:500 goat-anti rabbit 488 secondary antibody in 2% NGS
      • 2hr at RT
    • Wash 3 x 15 minutes in 1x PBST
    • Leave in 2% NGS at 4C over weekend.


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