User:Robert M. MacCallum/WTFGSB Reportback: Difference between revisions
(New page: =Welcome Trust Functional Genomics and Systems Biology Workshop= 30 November to 1 December 2009 ===Seth Grant=== Complexity of post-synaptic molecular machinery (several thousand prote...) |
No edit summary |
||
| Line 2: | Line 2: | ||
30 November to 1 December 2009 | 30 November to 1 December 2009 | ||
==Day one== | |||
===Edison Liu=== | |||
Estrogen (or is it EGF) receptor (ER) binding site analysis (ChIP and bioinf) - "Cosmic" score, correlation with RNA PolII binding and H3K4meX marks. | |||
Some functional binding is 1Mb away from gene!! | |||
Only 9% in 5k "promoter". | |||
Cool ChIA-PET (ChiA-seq) method to determine chromosomal loops. | |||
Looping for efficient transcription, grouping of coregulated genes ("looped out" genes don't respond to ER) | |||
==Day two== | |||
===Seth Grant=== | ===Seth Grant=== | ||
| Line 14: | Line 31: | ||
CNV in mouse | CNV in mouse | ||
What's special about pathological CNVs? (vs. benign) | |||
Human CNVs look up mouse phenotypes (somehow!) | |||
Enrichment! | |||
Revision as of 02:55, 1 December 2009
Welcome Trust Functional Genomics and Systems Biology Workshop
30 November to 1 December 2009
Day one
Edison Liu
Estrogen (or is it EGF) receptor (ER) binding site analysis (ChIP and bioinf) - "Cosmic" score, correlation with RNA PolII binding and H3K4meX marks.
Some functional binding is 1Mb away from gene!! Only 9% in 5k "promoter".
Cool ChIA-PET (ChiA-seq) method to determine chromosomal loops.
Looping for efficient transcription, grouping of coregulated genes ("looped out" genes don't respond to ER)
Day two
Seth Grant
Complexity of post-synaptic molecular machinery (several thousand proteins). Conserved in invertebrates (50% of prots) and single celled (25%). Evolution of the machinery (including plasticity) preceded evolution of synapses.
Very slow evolution.
Many diseases.
Caleb Webber
CNV in mouse
What's special about pathological CNVs? (vs. benign)
Human CNVs look up mouse phenotypes (somehow!)
Enrichment!
