User:Roberta Diaz Jimenez/Notebook/CHEM 472/2016/02/02: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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*Metal and Fructose Stock Solutions
*Metal and Fructose Stock Solutions
#Label three falcon tubes to make the concentrated solutions.  
#Label three falcon tubes to make the concentrated solutions.  
#


*Protein Stock Solutions
*Protein Stock Solutions
#Labelled falcon tubes for Casein, Whey, Soy, Rice, and Pea proteins.
#Labelled falcon tubes for Casein, Whey, Soy, Rice, and Pea proteins.
#Filled each tube with 10mLs of water.
#Added 5.78mg of casein into its tube.
#


*Sample Prep
*Sample Prep
#Label eppendorf tubes 1 through 64.
#Label eppendorf tubes 1 through 64.
#Follow the following table:
 
'''Table 1.''' Protocol to make gold and silver samples
*Detailed procedure can be found [https://docs.google.com/spreadsheets/d/1XIncoYXZyIeGlpAFkbnr44U3PQ61jNbax-hkexNerUM/edit#gid=303734626 SRB Lab Notebook Data] for this entry's date
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|''' Protein concentration'''
==Results==
| align="center" style="background:#f0f0f0;"|''' Sample # '''
*Detailed results can be found [https://docs.google.com/spreadsheets/d/1XIncoYXZyIeGlpAFkbnr44U3PQ61jNbax-hkexNerUM/edit#gid=303734626 SRB Lab Notebook Data] for this entry's date
| align="center" style="background:#f0f0f0;"|''' 2.5mM HAuCl4 (µL)'''
| align="center" style="background:#f0f0f0;"|''' 2.5mM AgNO3 (µL)'''
| align="center" style="background:#f0f0f0;"|''' Protein (µL)'''
| align="center" style="background:#f0f0f0;"|''' 2.5mM Fructose (µL)'''
| align="center" style="background:#f0f0f0;"|''' Water (µL)'''
| align="center" style="background:#f0f0f0;"|''' Au/Ag : Protein'''
| align="center" style="background:#f0f0f0;"|''' Notes'''
|-
|  ||1||40||0||560||0||400||1:15||


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Latest revision as of 01:29, 27 September 2017

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Objective

Set up reactions for our five proteins with silver and gold in order to obtain nanoparticles. Make stock solutions for both the metals, fructose, and the five proteins.

Procedure

  • Metal and Fructose Stock Solutions
  1. Label three falcon tubes to make the concentrated solutions.
  • Protein Stock Solutions
  1. Labelled falcon tubes for Casein, Whey, Soy, Rice, and Pea proteins.
  • Sample Prep
  1. Label eppendorf tubes 1 through 64.

Results


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