User:Roberta Diaz Jimenez/Notebook/CHEM 472/2016/02/02: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
Line 90: Line 90:
|-
|-
|22||0||40||200||40||720||
|22||0||40||200||40||720||
==Results==
*Detailed results can be found [https://docs.google.com/spreadsheets/d/1XIncoYXZyIeGlpAFkbnr44U3PQ61jNbax-hkexNerUM/edit#gid=303734626 SRB Lab Notebook Data] for this entry's date


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 14:20, 4 May 2016

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objective

Set up reactions for our five proteins with silver and gold in order to obtain nanoparticles. Make stock solutions for both the metals, fructose, and the five proteins.

Procedure

  • Metal and Fructose Stock Solutions
  1. Label three falcon tubes to make the concentrated solutions.
  • Protein Stock Solutions
  1. Labelled falcon tubes for Casein, Whey, Soy, Rice, and Pea proteins.
  2. Filled each tube with 10mLs of water.
  3. Added 5.78mg of casein into its tube.
  • Sample Prep
  1. Label eppendorf tubes 1 through 64.
  2. Follow the following tables:

Table 1. Protocol to make gold and silver samples using Casein as protein

Sample # 2.5mM HAuCl4 (µL) 2.5mM AgNO3 (µL) Protein (µL) 2.5mM Fructose (µL) Water (µL) Au/Ag : Protein Notes
1 40 0 560 0 400 1:15
2 40 0 440 0 520 1:11
3 40 0 360 0 600 1:09
4 40 0 280 0 680 1:07
5 40 0 200 0 760 1:05
6 0 40 560 0 600 1:15
7 0 40 360 0 580 1:09
8 0 40 200 0 560 1:05


Table 2. Protocol to make gold and silver samples using other five proteins

Sample # 2.5mM HAuCl4 (µL) 2.5mM AgNO3 (µL) Protein (µL) 2.5mM Fructose (µL) Water (µL) Au/Ag : Protein Notes
9 40 0 560 0 400 1:15
10 40 0 440 0 520 1:11
11 40 0 360 0 600 1:09
12 40 0 280 0 680 1:07
13 40 0 200 0 760 1:05
14 0 40 560 0 400
15 0 40 560 20 380
16 0 40 560 40 360
17 0 40 360 0 600
18 0 40 360 20 580
19 0 40 360 40 560
20 0 40 200 0 760
21 0 40 200 20 740
22 0 40 200 40 720

Results
Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Roberta_Diaz_Jimenez/Notebook/CHEM_472/2016/02/02&oldid=941097"