User:Roberta Diaz Jimenez/Notebook/CHEM 472/2016/03/29: Difference between revisions

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==Objective==
Data analysis for reactions run on March 15
==Data Workup protocol for all Main UV-VIS Machine analysis and Fluorescence Spectroscopy==
UVVis
#import data into excel
#Blank sample data by subtracting water blank absorbance values for before and after reaction
#Absorbance against wavelength
# Monitor maximum absorbance wavelengths before and after
#monitor absorbance wavelengths
Fluorescence
#import data into excel
#subtract water blank sample data from nanoparticle sample data (before and after)
#compute integral for each sample from between 305nm-470nm (peak size and cut off ends to avoid noise in integral calculation)
# calculate ratios of before and after of integrals for same same (just before and after)
# monitor fluorescence peak emission wavelengths


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Revision as of 14:01, 4 May 2016

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Objective

Data analysis for reactions run on March 15

Data Workup protocol for all Main UV-VIS Machine analysis and Fluorescence Spectroscopy

UVVis

  1. import data into excel
  2. Blank sample data by subtracting water blank absorbance values for before and after reaction
  3. Absorbance against wavelength
  4. Monitor maximum absorbance wavelengths before and after
  5. monitor absorbance wavelengths

Fluorescence

  1. import data into excel
  2. subtract water blank sample data from nanoparticle sample data (before and after)
  3. compute integral for each sample from between 305nm-470nm (peak size and cut off ends to avoid noise in integral calculation)
  4. calculate ratios of before and after of integrals for same same (just before and after)
  5. monitor fluorescence peak emission wavelengths



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