User:Roberta Diaz Jimenez/Notebook/CHEM 472/2016/04/12

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Objective

  1. Prepare 20mL AgNP and AuNP reactions for all five proteins: casein, soy, whey, rice, and pea.
  2. Conduct UV/Vis and Fluorescence analysis before placing samples in the oven for reaction to proceed.
  • Note: standardized silver reaction protein concentrations were used, as opposed to previous concentrations where casein had a larger concentration. This was deemed not necessary to obtain successful results.

Materials

  1. Used same nutritional proteins as used in all previous reaction sample preps.
  2. Protein and gold stocks prepared fresh every day.
  3. Main UV/Vis machine was used to record UV/VIS Spectra of Casein AgNPs, not ocean optics.
    1. A water blank in the same quartz cuvette was subtracted from the spectra before running any samples.

Procedure

Data


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