User:Roberta Diaz Jimenez/Notebook/CHEM 472/2016/04/13

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Objective

Perform a fifth disc diffusion assay using newly made metal-protein NP samples.

Materials

  1. Mueller Hinton Agar self-prepared (March 2)tored in fridge
  2. Whatman Filter Paper (6mm discs punched out from larger discs)*
    1. Switched from Whatman membrane filter paper discs, to these which are much more absorbent, and show no leakage whatsoever, we probably should have been using these from the beginning
  3. Autoclaved in dry cycle
  4. Dilutions of Protein Nanoparticle Samples and Ampicillin Control
    1. Used the metal-protein nanoparticle solutions synthesized on April 12.

Methods

  1. Silver and gold protein nanoparticle dilutions performed following the dilution factors below:
Concentration (x)
1
0.5
0.25
0.125
0.0625
  1. AMP control dilutions
Concentration (mg/L) Concentration (ug/uL) Volume (ul) Mass (ug)
4 0.004 25 0.1
8 0.008 25 0.2
16 0.016 25 0.4
32 0.032 25 0.8
64 0.064 25 1.6

Results


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